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Amperometric uric acid sensors based on polyelectrolyte multilayer films.
MedLine Citation:
PMID:  18969195     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Uricase (UOx) and polyelectrolyte were used for preparation of a permselective multilayer film and enzyme multilayer films on a platinum (Pt) electrode, allowing the detection of uric acid amperometrically. The polyelectrolyte multilayer (PEM) film composed of poly(allylamine) (PAA) and poly(vinyl sulfate) (PVS) were prepared via layer-by-layer assembly on the electrode, functioning as H(2)O(2)-selective film. After deposition of the permselective film (PAA/PVS)(2)PAA, UOx and PAA were deposited via layer-by-layer sequential deposition up to 10 UOx layers to prepare amperometric sensors for uric acid. Current response to uric acid was recorded at +0.6 V vs. Ag/AgCl to detect H(2)O(2) produced from the enzyme reaction. The response current increased with increasing the number of UOx layers. Even in the presence of ascorbic acid, uric acid can be detected over the concentration range 10(-6)-10(-3) M. The response current and deposited amount of UOx were affected by deposition bath pH and the addition of salt. The deposition of PAA/UOx film prepared in 2 mg ml(-1) solution (pH 11) of PAA with NaCl (8 mg ml(-1)) and 0.1 mg ml(-1) solution (pH 8.5) of UOx with borate (100 mM) resulted in an electrode which shows the largest response to uric acid. The response of the sensor to uric acid was decreased by 40% from the original activity after 30 days.
Authors:
Tomonori Hoshi; Hidekazu Saiki; Jun-Ichi Anzai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Talanta     Volume:  61     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2008-10-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  363-8     Citation Subset:  -    
Affiliation:
Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki Aoba-ku, Sendai 980-8578, Japan.
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