Document Detail


Amperometric measurements of catecholamine release from single vesicles in MN9D cells.
MedLine Citation:
PMID:  19094057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MN9D cells have been used as a successful model to investigate dopamine pharmacology and to test the specific effects of drugs for the treatment of Parkinson's disease. However, quantitative measurements of quantal release from these cells have not been carried out. In this work, we used amperometry to investigate catecholamine release from MN9D cells. Amperometric events were observed in both undifferentiated and differentiated (butyric acid-treated) cells. An increase in quantal size and half-width was observed for differentiated cells versus undifferentiated cells; however, the number of events per cell and the amplitude remained constant. In transmission electron microscopy images, no obvious cluster of small synaptic vesicles was observed, and large dense-core vesicles were present in the cell body of undifferentiated cells; however, after differentiation, vesicles were concentrated in the cell processes. In differentiated cells, l-DOPA caused an increase in quantal size and half-width, which could be blocked by the vesicular monoamine transporter inhibitor, reserpine.
Authors:
Yan Dong; Michael L Heien; Marc M Maxson; Andrew G Ewing
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  107     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-19     Completed Date:  2009-04-09     Revised Date:  2013-06-04    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  1589-95     Citation Subset:  IM    
Affiliation:
Department of Chemistry, The Pennsylvania State University, University Park, PA, USA.
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MeSH Terms
Descriptor/Qualifier:
Adrenergic Uptake Inhibitors / pharmacology
Animals
Catecholamines / analysis*
Cell Differentiation / drug effects,  physiology*
Cell Line, Transformed / metabolism*,  ultrastructure
Dopamine Agents / pharmacology
Electrochemical Techniques / methods*
Exocytosis / drug effects,  physiology
Levodopa / pharmacology
Mice
Microscopy, Electron, Transmission / methods
Potassium Chloride / pharmacology
Reserpine / pharmacology
Secretory Vesicles / metabolism,  ultrastructure
Grant Support
ID/Acronym/Agency:
R01 EB000352-10/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Adrenergic Uptake Inhibitors; 0/Catecholamines; 0/Dopamine Agents; 0/Levodopa; 50-55-5/Reserpine; 7447-40-7/Potassium Chloride
Comments/Corrections

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