| Amperometric glucose biosensor with remarkable acid stability based on glucose oxidase entrapped in colloidal gold-modified carbon ionic liquid electrode. | |
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MedLine Citation:
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PMID: 20510599 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A colloidal gold-modified carbon ionic liquid electrode was constructed by mixing colloidal gold-modified graphite powder with a solid room temperature ionic liquid n-octyl-pyridinium hexafluorophosphate (OPPF(6)). Glucose oxidase (GOD) was entrapped in this composite matrix and maintained its bioactivity well and displayed excellent stability. The effect conditions of pH, applied potential and GOD loading were examined. Especially, the glucose oxidase entrapped in this carbon ionic liquid electrode fully retained its activity upon stressing in strongly acidic conditions (pH 2.0) for over one hour. The proposed biosensor responds to glucose linearly over concentration range of 5.0x10(-6) to 1.2x10(-3) and 2.6x10(-3) to 1.3x10(-2) M, and the detection limit is 3.5x10(-6) M. The response time of the biosensor is fast (within 10s), and the life time is over two months. The effects of electroactive interferents, such as ascorbic acid, uric acid, can be significantly reduced by a Nafion film casting on the surface of resulting biosensor. |
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Authors:
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Xiaoying Liu; Xiandong Zeng; Nannan Mai; Yong Liu; Bo Kong; Yonghong Li; Wanzhi Wei; Shenglian Luo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-05 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 25 ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-06-23 Completed Date: 2010-10-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 2675-9 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Hunan, Changsha, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques
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methods* Carbon Electrochemical Techniques Enzymes, Immobilized* Glucose / analysis* Glucose Oxidase* Gold Colloid Graphite Hydrogen-Ion Concentration Ionic Liquids Limit of Detection |
| Chemical | |
Reg. No./Substance:
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0/Enzymes, Immobilized; 0/Gold Colloid; 0/Ionic Liquids; 50-99-7/Glucose; 7440-44-0/Carbon; 7782-42-5/Graphite; EC 1.1.3.4/Glucose Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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