Document Detail


Amperometric determination of underivatized amino acids at a nickel-modified gold electrode by anion-exchange chromatography.
MedLine Citation:
PMID:  10843545     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A nickel-based composite electrode obtained by anodic electrodeposition of nickel (III) oxyhydroxide film on the gold electrode substrate was characterized as an amperometric sensor and successfully applied to the determination of underivatised amino acids in flow-through systems. The electrodeposition of nickel oxyhydroxide films was obtained by cycling a gold electrode between 0.0 V and +1.0 V vs. a saturated calomel electrode in a 80 microM Ni2+ solution buffered at pH 10 with NaHCO3/Na2CO3. The resulting Au-Ni composite electrode exhibits good stability in alkaline medium and can be used as an amperometric sensor of underivatised amino acids at a fixed applied potential (+0.55 V vs. Ag/AgCl). The detection limits (S/N=3) for all investigated compounds ranged between 5 and 30 pmol injected, while the linear ranges spanned over two or three orders of magnitude. The contents of several free amino acids in two sample cheeses from different brands were evaluated by calibration graphs.
Authors:
I G Casella; M Gatta; T R Cataldi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  878     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-09-07     Completed Date:  2000-09-07     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  57-67     Citation Subset:  IM    
Affiliation:
Dipartimento di Chimica, Università degli Studi della Basilicata, Potenza, Italy. casella@unibas.it
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Anion Exchange Resins
Chromatography, Ion Exchange / methods*
Electrochemistry / methods*
Electrodes*
Gold / chemistry
Nickel / chemistry
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Anion Exchange Resins; 7440-02-0/Nickel; 7440-57-5/Gold

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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