| Amperometric determination of underivatized amino acids at a nickel-modified gold electrode by anion-exchange chromatography. | |
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MedLine Citation:
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PMID: 10843545 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A nickel-based composite electrode obtained by anodic electrodeposition of nickel (III) oxyhydroxide film on the gold electrode substrate was characterized as an amperometric sensor and successfully applied to the determination of underivatised amino acids in flow-through systems. The electrodeposition of nickel oxyhydroxide films was obtained by cycling a gold electrode between 0.0 V and +1.0 V vs. a saturated calomel electrode in a 80 microM Ni2+ solution buffered at pH 10 with NaHCO3/Na2CO3. The resulting Au-Ni composite electrode exhibits good stability in alkaline medium and can be used as an amperometric sensor of underivatised amino acids at a fixed applied potential (+0.55 V vs. Ag/AgCl). The detection limits (S/N=3) for all investigated compounds ranged between 5 and 30 pmol injected, while the linear ranges spanned over two or three orders of magnitude. The contents of several free amino acids in two sample cheeses from different brands were evaluated by calibration graphs. |
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Authors:
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I G Casella; M Gatta; T R Cataldi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. A Volume: 878 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2000 May |
Date Detail:
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Created Date: 2000-09-07 Completed Date: 2000-09-07 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 57-67 Citation Subset: IM |
Affiliation:
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Dipartimento di Chimica, Università degli Studi della Basilicata, Potenza, Italy. casella@unibas.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Anion Exchange Resins Chromatography, Ion Exchange / methods* Electrochemistry / methods* Electrodes* Gold / chemistry Nickel / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Anion Exchange Resins; 7440-02-0/Nickel; 7440-57-5/Gold |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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