Document Detail


Amniotic lamellar body counts determined with the Sysmex XE-2100 analyzer to predict fetal lung maturity during diabetic and other complicated pregnancies.
MedLine Citation:
PMID:  20509824     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The detection of amniotic lamellar bodies (LB) has been shown to be a rapid and simple way to assess fetal lung maturity (FLM). The maturity thresholds for LB vary due to different factors, one being the type of particle-count analyser used.
MATERIAL AND METHODS: The Sysmex XE-2100 hematological analyser was evaluated in determination of amniotic LB counts and compared with lecithin/sphingomyelin (L/S) and phosphatidylglycerol (PG) determination. We analysed 132 amniotic samples from a total of 109 mothers (71 diabetic) with 112 infants. Results. The correlation between the LB counts obtained with the Sysmex XE-2100 and our reference thin layer chromatography (TLC) phospholipid method was good. Samples with low L/S ratio (< or = 2.0) and no PG (i.e. premature fetal lung status), had low LB counts (n = 18, mean 8500/L, range 1000-26000), whereas 51 samples with mature fetal lung status had high LB counts (mean 63600/uL, range 20,000-139,000). In all our four cases of respiratory distress syndrome the LB counts were low (range 1000-28000/uL). The reference values for FLM determination were established: < or = 6000/microL for immature, values between 7000 and 35,000/uL for borderline results and >35,000/uL for mature. CONCLUSIONS. The amniotic LB count analysis with Sysmex XE-2100 has many advantages being a repeatable, inexpensive and quantitative method with a very short turn-around time. Consequently, our routine is to perform LB counts initially from all amniotic samples and only borderline LB results are analysed with TLC.
Authors:
Lotta Joutsi-Korhonen; Ansa Aitokallio-Tallberg; Erja Halmesmäki; Esa Hämäläinen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation     Volume:  70     ISSN:  1502-7686     ISO Abbreviation:  Scand. J. Clin. Lab. Invest.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-24     Completed Date:  2010-11-24     Revised Date:  2013-05-20    
Medline Journal Info:
Nlm Unique ID:  0404375     Medline TA:  Scand J Clin Lab Invest     Country:  England    
Other Details:
Languages:  eng     Pagination:  358-63     Citation Subset:  IM    
Affiliation:
HUSLAB Laboratory Services, Clinical Chemistry and Hematology, Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Adult
Amniotic Fluid / chemistry*
Cytological Techniques / instrumentation
Female
Fetal Organ Maturity*
Humans
Infant, Newborn
Lecithins / analysis
Lung / embryology*
Male
Phosphatidylglycerols / analysis
Pregnancy
Pregnancy Complications / physiopathology*
Pregnancy in Diabetics / physiopathology*
Pulmonary Surfactants / analysis*
Respiratory Distress Syndrome, Newborn / diagnosis
Sphingomyelins / analysis
Chemical
Reg. No./Substance:
0/Lecithins; 0/Phosphatidylglycerols; 0/Pulmonary Surfactants; 0/Sphingomyelins

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