| Amniotic lamellar body counts determined with the Sysmex XE-2100 analyzer to predict fetal lung maturity during diabetic and other complicated pregnancies. | |
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MedLine Citation:
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PMID: 20509824 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The detection of amniotic lamellar bodies (LB) has been shown to be a rapid and simple way to assess fetal lung maturity (FLM). The maturity thresholds for LB vary due to different factors, one being the type of particle-count analyser used. MATERIAL AND METHODS: The Sysmex XE-2100 hematological analyser was evaluated in determination of amniotic LB counts and compared with lecithin/sphingomyelin (L/S) and phosphatidylglycerol (PG) determination. We analysed 132 amniotic samples from a total of 109 mothers (71 diabetic) with 112 infants. Results. The correlation between the LB counts obtained with the Sysmex XE-2100 and our reference thin layer chromatography (TLC) phospholipid method was good. Samples with low L/S ratio (< or = 2.0) and no PG (i.e. premature fetal lung status), had low LB counts (n = 18, mean 8500/L, range 1000-26000), whereas 51 samples with mature fetal lung status had high LB counts (mean 63600/uL, range 20,000-139,000). In all our four cases of respiratory distress syndrome the LB counts were low (range 1000-28000/uL). The reference values for FLM determination were established: < or = 6000/microL for immature, values between 7000 and 35,000/uL for borderline results and >35,000/uL for mature. CONCLUSIONS. The amniotic LB count analysis with Sysmex XE-2100 has many advantages being a repeatable, inexpensive and quantitative method with a very short turn-around time. Consequently, our routine is to perform LB counts initially from all amniotic samples and only borderline LB results are analysed with TLC. |
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Authors:
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Lotta Joutsi-Korhonen; Ansa Aitokallio-Tallberg; Erja Halmesmäki; Esa Hämäläinen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Scandinavian journal of clinical and laboratory investigation Volume: 70 ISSN: 1502-7686 ISO Abbreviation: Scand. J. Clin. Lab. Invest. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-24 Completed Date: 2010-11-24 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 0404375 Medline TA: Scand J Clin Lab Invest Country: England |
Other Details:
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Languages: eng Pagination: 358-63 Citation Subset: IM |
Affiliation:
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HUSLAB Laboratory Services, Clinical Chemistry and Hematology, Helsinki, Finland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Amniotic Fluid / chemistry* Cytological Techniques / instrumentation Female Fetal Organ Maturity* Humans Infant, Newborn Lecithins / analysis Lung / embryology* Male Phosphatidylglycerols / analysis Pregnancy Pregnancy Complications / physiopathology* Pregnancy in Diabetics / physiopathology* Pulmonary Surfactants / analysis* Respiratory Distress Syndrome, Newborn / diagnosis Sphingomyelins / analysis |
| Chemical | |
Reg. No./Substance:
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0/Lecithins; 0/Phosphatidylglycerols; 0/Pulmonary Surfactants; 0/Sphingomyelins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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