Document Detail

Amniotic fluid volume and composition in mouse pregnancy.
MedLine Citation:
PMID:  16325744     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: The current study was undertaken to determine simultaneous changes in amniotic fluid (AF) volume and composition across gestation in the pregnant mouse. METHODS: Young adult mice (6 to 7 weeks old) of the CB6F1 strain were mated overnight. AF was collected on consecutive days from embryonic days 9.5 through 18.5 for measurements of volume and composition. Statistical analysis included one-factor analysis of variance (ANOVA). RESULTS: AF volume increased from 18 +/- 4 (SE) microL on day 9.5 to a maximum of 147 +/- 4 microL on days 15.5 to 16.5 and decreased sharply to 17 +/- 3 microL on day 18.5. AF osmolality was unchanged except for a rise prior to delivery on day 19.5 to 20.5. AF sodium, calcium, and glucose concentrations increased and subsequently decreased as gestation progressed. AF potassium, chloride, and lactate concentrations initially decreased and then increased across gestation. Prior to day 9.5 and after day 18.5, AF volume was too small for volume or compositional determinations. CONCLUSIONS: In the mouse, the rise in AF volume from mid gestation to a maximum late in gestation is similar to that in humans while the sharp fall prior to delivery is not. As observed in the fetal sheep, the changes in fluid volume are associated with AF osmolality and solute concentration changes that are correlated with advancing gestational age. These observations together with the feasibility of quantifying AF volume and composition in the mouse fetus demonstrate the possibility of using genetically altered mice as a model for future studies on the molecular mechanisms underlying the regulation of AF volume and composition.
Cecilia Y Cheung; Robert A Brace
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of the Society for Gynecologic Investigation     Volume:  12     ISSN:  1556-7117     ISO Abbreviation:  J. Soc. Gynecol. Investig.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-05     Completed Date:  2006-04-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9433806     Medline TA:  J Soc Gynecol Investig     Country:  United States    
Other Details:
Languages:  eng     Pagination:  558-62     Citation Subset:  IM    
Division of Perinatal Medicine, Department of Reproductive Medicine, University of California San Diego, La Jolla, California 92093-0802, USA.
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MeSH Terms
Amniotic Fluid* / chemistry,  physiology
Calcium / analysis
Chlorides / analysis
Gestational Age
Glucose / analysis
Lactic Acid / analysis
Mice, Inbred Strains
Models, Animal
Osmolar Concentration
Potassium / analysis
Sodium / analysis
Grant Support
Reg. No./Substance:
0/Chlorides; 50-21-5/Lactic Acid; 50-99-7/Glucose; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium

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