| Amniotic fluid volume and composition in mouse pregnancy. | |
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MedLine Citation:
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PMID: 16325744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The current study was undertaken to determine simultaneous changes in amniotic fluid (AF) volume and composition across gestation in the pregnant mouse. METHODS: Young adult mice (6 to 7 weeks old) of the CB6F1 strain were mated overnight. AF was collected on consecutive days from embryonic days 9.5 through 18.5 for measurements of volume and composition. Statistical analysis included one-factor analysis of variance (ANOVA). RESULTS: AF volume increased from 18 +/- 4 (SE) microL on day 9.5 to a maximum of 147 +/- 4 microL on days 15.5 to 16.5 and decreased sharply to 17 +/- 3 microL on day 18.5. AF osmolality was unchanged except for a rise prior to delivery on day 19.5 to 20.5. AF sodium, calcium, and glucose concentrations increased and subsequently decreased as gestation progressed. AF potassium, chloride, and lactate concentrations initially decreased and then increased across gestation. Prior to day 9.5 and after day 18.5, AF volume was too small for volume or compositional determinations. CONCLUSIONS: In the mouse, the rise in AF volume from mid gestation to a maximum late in gestation is similar to that in humans while the sharp fall prior to delivery is not. As observed in the fetal sheep, the changes in fluid volume are associated with AF osmolality and solute concentration changes that are correlated with advancing gestational age. These observations together with the feasibility of quantifying AF volume and composition in the mouse fetus demonstrate the possibility of using genetically altered mice as a model for future studies on the molecular mechanisms underlying the regulation of AF volume and composition. |
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Authors:
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Cecilia Y Cheung; Robert A Brace |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of the Society for Gynecologic Investigation Volume: 12 ISSN: 1556-7117 ISO Abbreviation: J. Soc. Gynecol. Investig. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-12-05 Completed Date: 2006-04-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9433806 Medline TA: J Soc Gynecol Investig Country: United States |
Other Details:
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Languages: eng Pagination: 558-62 Citation Subset: IM |
Affiliation:
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Division of Perinatal Medicine, Department of Reproductive Medicine, University of California San Diego, La Jolla, California 92093-0802, USA. cycheung@ucsd.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid*
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chemistry,
physiology Animals Calcium / analysis Chlorides / analysis Female Fetus Gestational Age Glucose / analysis Lactic Acid / analysis Mice Mice, Inbred Strains Models, Animal Osmolar Concentration Potassium / analysis Pregnancy Sodium / analysis |
| Grant Support | |
ID/Acronym/Agency:
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HD39240/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 50-21-5/Lactic Acid; 50-99-7/Glucose; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium |
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