| Amniotic fluid ferritin as a marker of intestinal damage in gastroschisis: a time course experimental study. | |
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MedLine Citation:
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PMID: 17923200 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/PURPOSE: Intestinal damage (ID) is closely related to morbidity and mortality in gastroschisis. This study was performed to determine the intraamniotic substances that may correlate ID and also to verify their time course levels that would be useful for determining when ID starts in gastroschisis. METHODS: In this study, 13-day-old fertilized chick eggs were used. The amnioallantoic membrane was perforated to create amnioallantoic cavity in all embryos. Gastroschisis was created in gastroschisis group to simulate human gastroschisis. Amnioallantoic fluid samples were collected from the embryos on the 13th to 19th gestational days, and the intestines of each group were harvested for evaluation. Amnioallantoic levels of interleukin-8, ferritin, alkaline phosphatase, and amylase were measured. Serosal thickness of the intestines in each group was evaluated. RESULTS: Increasing amnioallantoic fluid levels of interleukin-8, alkaline phosphatase, and amylase were found in both groups. In contrast to control group, ferritin levels, as a sign of inflammation, were found increased only in gastroschisis group. Histopathologic examination of intestines in the gastroschisis group showed a significant increase in the serosal thickness especially after the 16th day. CONCLUSION: Increases in amnioallantoic fluid levels of ferritin show promise as a marker for determining ID encountered in gastroschisis but warrant further investigation. |
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Authors:
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Meltem Cağlar; Gülce Hakgüder; Oğuz Ateş; Mustafa Olguner; Canan Coker; Erdener Ozer; Feza M Akgür |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pediatric surgery Volume: 42 ISSN: 1531-5037 ISO Abbreviation: J. Pediatr. Surg. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-10-09 Completed Date: 2007-10-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0052631 Medline TA: J Pediatr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1710-5 Citation Subset: IM |
Affiliation:
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Department of Pediatric Surgery, Dokuz Eylül University, Medical School, 35340 Izmir, Turkey. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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analysis Amniotic Fluid / chemistry* Amylases / analysis Animals Biological Markers Chick Embryo Disease Models, Animal Ferritins / analysis* Gastroschisis / etiology, metabolism*, pathology Inflammation Interleukin-8 / analysis Intestines / embryology, ultrastructure Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Interleukin-8; 9007-73-2/Ferritins; EC 3.1.3.1/Alkaline Phosphatase; EC 3.2.1.-/Amylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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