Document Detail


Amniotic fluid amino acid concentrations are modified by maternal dietary glucose, gestational age, and fetal growth in rats.
MedLine Citation:
PMID:  16140901     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amniotic fluid (AF) contains free amino acids that enter via transplacental and transmembranous routes from maternal sources; subsequently, the developing fetus "ingests" these amino acids early in gestation through unkeratinized skin and later through continuous AF swallowing. Our objectives were as follows: 1) to determine whether a restriction of maternal dietary glucose modulates the free AF amino acid pool, and 2) to establish whether any diet-induced changes were predictive of fetal weight near term (d 21.5). To produce varying in utero growth rates, pregnant rat dams were fed varying levels of glucose (0, 12, 24, 60%) throughout pregnancy. AF samples, collected on gestational days 18-21, were precolumn derivatized by 9-fluorenylmethyloxychloroformate to produce stable primary and secondary amino acid derivatives required for HPLC detection at low amino acid concentrations. Eighteen amino acids were identified. A 2-way ANOVA with main effects of diet (< or =12% and > or =24% glucose) and gestational age (d 18/19 and 20/21) showed that 2 AF amino acids, methionine and phenylalanine, and 12 AF amino acids were independently modified by diet and gestational age, respectively. Of note were the 364% increase in AF methionine and the constant decline in AF taurine as both gestational age lengthened and fetal weight increased. Multiple regression demonstrated that in addition to methionine, 3 specific AF amino acids, cysteine, lysine, and tyrosine, predicted fetal weight. These results demonstrate that the AF amino acid pool can be modified by the glucose content of the maternal diet and that specific AF amino acids are associated with gestational age and fetal growth.
Authors:
Christine N Gurekian; Kristine G Koski
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  135     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-05     Completed Date:  2005-11-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2219-24     Citation Subset:  IM    
Affiliation:
School of Dietetics and Human Nutrition, McGill University Macdonald Campus, Montreal, QC, Canada H9X 3V9.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Amniotic Fluid / metabolism*
Animals
Diet
Female
Fetal Development*
Gestational Age*
Glucose / administration & dosage*,  pharmacology
Methionine / metabolism
Osmolar Concentration
Phenylalanine / metabolism
Predictive Value of Tests
Pregnancy
Pregnancy, Animal / metabolism*
Rats
Taurine / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 107-35-7/Taurine; 50-99-7/Glucose; 63-68-3/Methionine; 63-91-2/Phenylalanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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