Document Detail

Ammonium persulfate: a safe alternative oxidizing reagent for measuring urinary iodine.
MedLine Citation:
PMID:  8595717     Owner:  NLM     Status:  MEDLINE    
The chloric acid method is most commonly used to obtain accurate and reproducible measurements of iodine and remove interfering substances. Unfortunately chloric acid is a potential hazard, requiring an explosion-proof hood, among other precautions. We have developed a simple, convenient, and economic method for measuring urinary iodine by using 1 mol/L ammonium persulfate, a nonexplosive, nonhazardous chemical, as the oxidizing reagent. The oxidation procedure can be completed in 30 min at a temperature of 91-95 degrees C. The iodine in the urine is then measured by a modification of the traditional colorimetric method of Sandell and Kolthoff. Urine samples (110) collected from a mixed population of healthy males and females, ranging in age from 6 to 79 years and living in the US, were analyzed for urine iodine content by two methods: the proposed ammonium persulfate method and the chloric acid method. The ammonium persulfate method has an intraassay CV of 9.1% at 0.42 +/- 0.04 micromol/L (mean +/- SD), 7.8% at 1.46 +/- 0.11 micromol/L, and 4.0% at 3.54 +/- 0.14 micromol/L. The interassay CV is 10.2% at 0.46 +/- 0.05 micromol/L, and 7.9% at 3.27 +/- 0.26 micromol/L. Recovery of iodine added to urine in vitro was 107%, 94%, and 97% for 0.42 micromol/L, 0.77 micromol/L and 3.64 micromol/L, respectively. The lower limit of detectability was 0.0034 microgram of iodine. Values for iodine in 110 urines measured by the reference chloric acid method ranged from 0.06 to 8.03 micromol/L and by the ammonium persulfate method from 0.05 to 7.4 micromol/L. The persulfate method (y) correlated extremely closely with the reference chloric acid method (x) by the Pearson correlation (y = 0.923x + 0.810 micromol/L, and r = 0.994, Sy/x = 1.841).
S Pino; S L Fang; L E Braverman
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical chemistry     Volume:  42     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-04-15     Completed Date:  1996-04-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  239-43     Citation Subset:  IM    
Division of Endocrinology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
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MeSH Terms
Ammonium Sulfate*
Colorimetry / methods*
Indicators and Reagents
Iodine / urine*
Reference Values
Sensitivity and Specificity
Grant Support
Reg. No./Substance:
0/Chlorates; 0/Indicators and Reagents; 0/Oxidants; 7553-56-2/Iodine; 7727-54-0/ammonium peroxydisulfate; 7783-20-2/Ammonium Sulfate

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