| Ammonium nitrate in the culture medium influences regeneration potential of cryopreserved shoot tips of Holostemma annulare. | |
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MedLine Citation:
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PMID: 11912508 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Influence of NH4NO3 in the pre-freeze and post-freeze culture medium and 2 or 30 day preconditioning in the presence of 0.5 M sucrose on regeneration of shoot tips of Holostemma annulare following cryopreservation using an encapsulation-dehydration protocol was studied. A long preconditioning phase of 30 days significantly reduced tissue water and improved post-freeze recovery of shoot tips. Under the long preconditioning treatment, Murashige & Skoog (MS) medium free of NH4NO3 (MS-3) allowed maximum regeneration (59%) of liquid nitrogen (LN) exposed shoot tips with less frequency of callusing (10.4%) after 45 days of post-freeze culture. Corresponding desiccated control shoot tips showed 85-90% regeneration. A 3.75 mM NH4NO3 concentration (MS-4) favoured 72-89% and 43-47% regeneration after desiccation and LN exposure respectively. The standard MS medium with 20.6 mM NH4NO3 (MS-1) allowed poor regeneration after desiccation (39-53%) as well as LN exposure (8-23%). The study reveals the importance of reducing ammonium nitrate in the culture medium to get maximum recovery of cryopreserved shoot tips of Holostemma annulare. |
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Authors:
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S William Decruse; S Seeni |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cryo letters Volume: 23 ISSN: 0143-2044 ISO Abbreviation: Cryo Letters Publication Date: 2002 Jan-Feb |
Date Detail:
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Created Date: 2002-03-25 Completed Date: 2002-05-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9891832 Medline TA: Cryo Letters Country: England |
Other Details:
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Languages: eng Pagination: 55-60 Citation Subset: IM |
Affiliation:
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Plant Biotechnology Division, Tropical Botanic Garden & Research Institute, Palode, Thiruvananthapuram-695562, Kerala, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Asclepiadaceae
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physiology* Butyric Acid / pharmacology Cryopreservation / methods* Cryoprotective Agents / pharmacology Culture Media / pharmacology* Desiccation Nitrates / pharmacology* Plant Shoots / chemistry, drug effects, growth & development Regeneration / drug effects* Water / analysis |
| Chemical | |
Reg. No./Substance:
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0/Cryoprotective Agents; 0/Culture Media; 0/Nitrates; 107-92-6/Butyric Acid; 6484-52-2/ammonium nitrate; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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