Document Detail

Ammonium nitrate in the culture medium influences regeneration potential of cryopreserved shoot tips of Holostemma annulare.
MedLine Citation:
PMID:  11912508     Owner:  NLM     Status:  MEDLINE    
Influence of NH4NO3 in the pre-freeze and post-freeze culture medium and 2 or 30 day preconditioning in the presence of 0.5 M sucrose on regeneration of shoot tips of Holostemma annulare following cryopreservation using an encapsulation-dehydration protocol was studied. A long preconditioning phase of 30 days significantly reduced tissue water and improved post-freeze recovery of shoot tips. Under the long preconditioning treatment, Murashige & Skoog (MS) medium free of NH4NO3 (MS-3) allowed maximum regeneration (59%) of liquid nitrogen (LN) exposed shoot tips with less frequency of callusing (10.4%) after 45 days of post-freeze culture. Corresponding desiccated control shoot tips showed 85-90% regeneration. A 3.75 mM NH4NO3 concentration (MS-4) favoured 72-89% and 43-47% regeneration after desiccation and LN exposure respectively. The standard MS medium with 20.6 mM NH4NO3 (MS-1) allowed poor regeneration after desiccation (39-53%) as well as LN exposure (8-23%). The study reveals the importance of reducing ammonium nitrate in the culture medium to get maximum recovery of cryopreserved shoot tips of Holostemma annulare.
S William Decruse; S Seeni
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  23     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2002 Jan-Feb
Date Detail:
Created Date:  2002-03-25     Completed Date:  2002-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  55-60     Citation Subset:  IM    
Plant Biotechnology Division, Tropical Botanic Garden & Research Institute, Palode, Thiruvananthapuram-695562, Kerala, India.
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MeSH Terms
Asclepiadaceae / physiology*
Butyric Acid / pharmacology
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Culture Media / pharmacology*
Nitrates / pharmacology*
Plant Shoots / chemistry,  drug effects,  growth & development
Regeneration / drug effects*
Water / analysis
Reg. No./Substance:
0/Cryoprotective Agents; 0/Culture Media; 0/Nitrates; 107-92-6/Butyric Acid; 6484-52-2/ammonium nitrate; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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