| Ammonia excretion by the skin of zebrafish (Danio rerio) larvae. | |
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MedLine Citation:
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PMID: 18815227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanism of ammonia excretion in freshwater teleosts is not well understood. In this study, scanning ion-selective electrode technique was applied to measure H(+) and NH(4)(+) fluxes in specific cells on the skin of zebrafish larvae. NH(4)(+) extrusion was relatively high in H(+) pump-rich cells, which were identified as the H(+)-secreting ionocyte in zebrafish. Minor NH(4)(+) extrusion was also detected in keratinocytes and other types of ionocytes in larval skin. NH(4)(+) extrusion from the skin was tightly linked to acid secretion. Increases in the external pH and buffer concentration (5 mM MOPS) diminished H(+) and NH(4)(+) gradients at the larval surface. Moreover, coupled decreases in NH(4)(+) and H(+) extrusion were found in larvae treated with an H(+)-pump inhibitor (bafilomycin A1) or H(+)-pump gene (atp6v1a) knockdown. Knockdown of Rhcg1 with morpholino-oligonucleotides also decreased NH(4)(+) excretion. This study demonstrates ammonia excretion in epithelial cells of larval skin through an acid-trapping mechanism, and it provides direct evidence for the involvement of the H(+) pump and an Rh glycoprotein (Rhcg1) in ammonia excretion. |
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Authors:
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Tin-Han Shih; Jiun-Lin Horng; Pung-Pung Hwang; Li-Yih Lin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-24 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 295 ISSN: 0363-6143 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-08 Completed Date: 2009-02-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: C1625-32 Citation Subset: IM |
Affiliation:
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Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan, Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ammonia
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metabolism* Animals Cation Transport Proteins / metabolism H(+)-K(+)-Exchanging ATPase / metabolism Ion-Selective Electrodes Larva Proton Pumps / metabolism Skin / metabolism* Zebrafish / physiology* Zebrafish Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cation Transport Proteins; 0/Proton Pumps; 0/Rhcg1 protein, zebrafish; 0/Zebrafish Proteins; 7664-41-7/Ammonia; EC 3.6.1.10/H(+)-K(+)-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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