Document Detail

Amitriptyline and fluoxetine protect PC12 cells from cell death induced by hydrogen peroxide.
MedLine Citation:
PMID:  15944744     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the potential protective effects of amitriptyline and fluoxetine in a catecholamine cell model. METHODS: Cultured rat pheochromocytoma (PC12) cells were pretreated with amitriptyline or fluoxetine for 24 or 48 hours and were then subjected to neurotoxic insult (200 micromol/L hydrogen peroxide). Cell viability was determined by measurement of the reduction product of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT). The enzyme activity of superoxide dismutase (SOD) was determined by a commercial SOD assay kit. RESULTS: The decrease in cell viability induced by hydrogen peroxide was attenuated in PC12 cells pretreated with 100 micromol/L amitriptyline for 24 hours or with 50 micromol/L amitriptyline or 50 micromol/L fluoxetine for 48 hours. Pretreatment with either amitriptyline or fluoxetine was associated with increased SOD activity in PC12 cells. Inhibition of SOD activity with diethyldithiocarbamic acid reduced the cytoprotective action of fluoxetine. CONCLUSIONS: These data suggest that the neuroprotective actions of some antidepressants include the upregulation of SOD activity.
Nathan Kolla; Zelan Wei; J Steven Richardson; Xin-Min Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of psychiatry & neuroscience : JPN     Volume:  30     ISSN:  1180-4882     ISO Abbreviation:  J Psychiatry Neurosci     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-06-09     Completed Date:  2005-07-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9107859     Medline TA:  J Psychiatry Neurosci     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  196-201     Citation Subset:  IM    
Neuropsychiatry Research Unit, Department of Psychiatry, University of Saskatchewan.
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MeSH Terms
Amitriptyline / pharmacology*
Antidepressive Agents, Second-Generation / pharmacology*
Antidepressive Agents, Tricyclic / pharmacology*
Cell Survival / drug effects
Fluoxetine / pharmacology*
Hydrogen Peroxide / antagonists & inhibitors*,  toxicity*
Oxidants / toxicity*
PC12 Cells
Superoxide Dismutase / metabolism
Reg. No./Substance:
0/Antidepressive Agents, Second-Generation; 0/Antidepressive Agents, Tricyclic; 0/Antioxidants; 0/Oxidants; 50-48-6/Amitriptyline; 54910-89-3/Fluoxetine; 7722-84-1/Hydrogen Peroxide; EC Dismutase

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