| Amitriptyline and fluoxetine protect PC12 cells from cell death induced by hydrogen peroxide. | |
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MedLine Citation:
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PMID: 15944744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the potential protective effects of amitriptyline and fluoxetine in a catecholamine cell model. METHODS: Cultured rat pheochromocytoma (PC12) cells were pretreated with amitriptyline or fluoxetine for 24 or 48 hours and were then subjected to neurotoxic insult (200 micromol/L hydrogen peroxide). Cell viability was determined by measurement of the reduction product of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT). The enzyme activity of superoxide dismutase (SOD) was determined by a commercial SOD assay kit. RESULTS: The decrease in cell viability induced by hydrogen peroxide was attenuated in PC12 cells pretreated with 100 micromol/L amitriptyline for 24 hours or with 50 micromol/L amitriptyline or 50 micromol/L fluoxetine for 48 hours. Pretreatment with either amitriptyline or fluoxetine was associated with increased SOD activity in PC12 cells. Inhibition of SOD activity with diethyldithiocarbamic acid reduced the cytoprotective action of fluoxetine. CONCLUSIONS: These data suggest that the neuroprotective actions of some antidepressants include the upregulation of SOD activity. |
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Authors:
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Nathan Kolla; Zelan Wei; J Steven Richardson; Xin-Min Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of psychiatry & neuroscience : JPN Volume: 30 ISSN: 1180-4882 ISO Abbreviation: J Psychiatry Neurosci Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-06-09 Completed Date: 2005-07-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9107859 Medline TA: J Psychiatry Neurosci Country: Canada |
Other Details:
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Languages: eng Pagination: 196-201 Citation Subset: IM |
Affiliation:
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Neuropsychiatry Research Unit, Department of Psychiatry, University of Saskatchewan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amitriptyline
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pharmacology* Animals Antidepressive Agents, Second-Generation / pharmacology* Antidepressive Agents, Tricyclic / pharmacology* Antioxidants* Cell Survival / drug effects Fluoxetine / pharmacology* Hydrogen Peroxide / antagonists & inhibitors*, toxicity* Oxidants / toxicity* PC12 Cells Rats Superoxide Dismutase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antidepressive Agents, Second-Generation; 0/Antidepressive Agents, Tricyclic; 0/Antioxidants; 0/Oxidants; 50-48-6/Amitriptyline; 54910-89-3/Fluoxetine; 7722-84-1/Hydrogen Peroxide; EC 1.15.1.1/Superoxide Dismutase |
| Comments/Corrections | |
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