Document Detail

Amitosis requires γ-tubulin-mediated microtubule assembly in Tetrahymena thermophila.
MedLine Citation:
PMID:  21246753     Owner:  NLM     Status:  MEDLINE    
To reveal the molecular systems involved in the division of a cell and its contents during cell proliferation is one of the major subjects in cell biology. Although cytoskeletal organization during mitosis has been well studied, consensus on the molecular basis of amitosis has not been achieved. Here we adapted an immunofluorescence method and investigated the cellular localization of γ-tubulin and microtubules (MTs) in dividing Tetrahymena. Although the macronucleus (Mac) lacks a bipolar spindle, γ-tubulin and MTs are specifically detected in the dividing Mac and show a marked change in the pattern of localization. First, γ-tubulin and MTs appear in whole Mac, then, γ-tubulin gathers at the center of the Mac where the aster-like structure of MTs forms. On Mac expansion, MTs associated with numerous dots of γ-tubulin are reorganized into longitudinally arranged bundles, suggesting that the mutual sliding of each filament and polymerization of MTs may induce Mac expansion. Moreover, normal Mac expansion and equal segregation of the Mac are severely disturbed when γ-tubulin is shut off. We propose that γ-tubulin-mediated MT assembly is required in Mac amitosis of Tetrahymena.
Yasuharu Kushida; Kentaro Nakano; Osamu Numata
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Publication Detail:
Type:  Journal Article     Date:  2010-12-22
Journal Detail:
Title:  Cytoskeleton (Hoboken, N.J.)     Volume:  68     ISSN:  1949-3592     ISO Abbreviation:  Cytoskeleton (Hoboken)     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-19     Completed Date:  2011-04-25     Revised Date:  2014-07-24    
Medline Journal Info:
Nlm Unique ID:  101523844     Medline TA:  Cytoskeleton (Hoboken)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  89-96     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley-Liss, Inc.
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MeSH Terms
Cell Nucleus Division / physiology*
Macronucleus / metabolism*
Microtubules / metabolism*
Protozoan Proteins / metabolism*
Tetrahymena thermophila / cytology,  metabolism*
Tubulin / metabolism*
Reg. No./Substance:
0/Protozoan Proteins; 0/Tubulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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