Document Detail


Aminomethyl coumarin acetic acid: a new fluorescent labelling agent for proteins.
MedLine Citation:
PMID:  3096915     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new fluorescent protein labelling agent, 7-amino-4-methyl coumarin-3-acetic acid (AMCA), emits in the blue region (440-460 nm) on activation with UV light (350 nm). The active reagent is the N-hydroxysuccinimide ester which reacts with lysine residues under mild conditions to form photostable amide links. The Stokes shift of 100 nm compared to 30 nm for Fluorescein isothiocyanate (FITC) allows easy filter discrimination of exciting and emitting radiation. The agent has been demonstrated in use for fluorohistochemical examination of human kidney glomeruli, using the sandwich technique and compared with the same procedure using FITC-labelled antibodies. The good quantum yield coupled with convenient emission lines in the mercury spectrum allows photographic exposure time of fluorescent labelled sections to be reduced to a quarter of that required for a corresponding FITC conjugate. AMCA-immunoglobulin conjugates were not susceptible to photobleaching and have a storage life at -20 degrees C of more than two years.
Authors:
H Khalfan; R Abuknesha; M Rand-Weaver; R G Price; D Robinson
Related Documents :
16697515 - Direct observation of protein folding in nanoenvironments using a molecular ruler.
25435325 - Crystal structure of the human, fic-domain containing protein hype and implications for...
25450595 - Crystal structure of the essential mycobacterium tuberculosis phosphopantetheinyl trans...
17766345 - Singlet oxygen photosensitization by egfp and its chromophore hbdi.
6386605 - The bacteriophage mu gene b product is incorporated into the inner membrane of escheric...
16248585 - Determination of the grape invertase content (using pta-elisa) following various fining...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Histochemical journal     Volume:  18     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1987-01-22     Completed Date:  1987-01-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  497-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Basement Membrane / analysis
Coumarins*
Fluorescein-5-isothiocyanate
Fluoresceins
Fluorescent Antibody Technique
Fluorescent Dyes*
Humans
Immunoglobulin G
Kidney Glomerulus / analysis*
Proteins / analysis*
Thiocyanates
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Coumarins; 0/Fluoresceins; 0/Fluorescent Dyes; 0/Immunoglobulin G; 0/Proteins; 0/Thiocyanates; 106562-32-7/7-amino-4-methylcoumarin-3-acetic acid; 3326-32-7/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Enzyme histochemistry of human melanomas and pigmented naevi with special reference to alpha-D-manno...
Next Document:  Tissue-type plasminogen activator in rat adrenal medulla.