| Aminoimidazole carboxamide ribonucleotide ameliorates experimental autoimmune uveitis. | |
| | |
MedLine Citation:
|
PMID: 22562518 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: To investigate the anti-inflammatory effect of an adenosine monophosphate (AMP) analog, aminoimidazole carboxamide ribonucleotide (AICAR), in experimental autoimmune uveoretinitis (EAU). METHODS: C57BL/6 mice were injected daily with AICAR (200 mg/kg, intraperitoneally [IP]) from day 0, the day of interphotoreceptor retinoid-binding protein (IRBP) immunization, until day 21. The severity of uveitis was assessed clinically and histopathologically. T-cell proliferation and cytokine production of IFN-γ, IL-17, and IL-10 in response to IRBP stimulation were determined. In addition, regulatory T-cell (Treg) populations were measured. Co-stimulatory molecule expression (CD40, 80, 86, and I-Ab) on dendritic cells (DCs) in EAU and on bone marrow-derived dendritic cells (BMDCs) treated with AICAR was measured. RESULTS: AICAR treatment significantly reduced clinical and histologic severity of EAU as well as ocular cytokine production. An anti-inflammatory effect associated with the inhibition of T-cell proliferation and Th1 and Th17 cytokine production was observed. Increases in the Th2 response and Treg population were not observed with AICAR treatment. AICAR did significantly inhibit BMDC maturation by reducing co-stimulatory molecule expression. CONCLUSIONS: AICAR attenuates EAU by preventing generation of Ag-specific Th1 and Th17 cells. Impaired DC maturation may be an underlying mechanism for this anti-inflammatory effect observed with AICAR. |
| | |
Authors:
|
Jun Suzuki; Takeru Yoshimura; Marina Simeonova; Kimio Takeuchi; Yusuke Murakami; Yuki Morizane; Joan W Miller; Lucia Sobrin; Demetrios G Vavvas |
Related Documents
:
|
22611238 - Innate ifns and plasmacytoid dendritic cells constrain th2 cytokine responses to rhinov... 20022228 - Gastrointestinal lymphatics in health and disease. 22814248 - Inhibitory effects of human immunodeficiency virus gp120 and tat on cpg-a-induced infla... 18957108 - Signal transduction in cells of the immune system in microgravity. 12181748 - Propapoptotic effects of nf-kappab in lncap prostate cancer cells lead to serine protea... 15319868 - Induction of apoptosis and release of interleukin-1 beta by cell wall-associated 19-kda... |
Publication Detail:
|
Type: Comparative Study; Journal Article Date: 2012-06-28 |
Journal Detail:
|
Title: Investigative ophthalmology & visual science Volume: 53 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2012 |
Date Detail:
|
Created Date: 2012-06-29 Completed Date: 2012-09-18 Revised Date: 2013-04-01 |
Medline Journal Info:
|
Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 4158-69 Citation Subset: IM |
Affiliation:
|
Retina Service, Angiogenesis Laboratory, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts 02114, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aminoimidazole Carboxamide
/
administration & dosage,
analogs & derivatives*,
therapeutic use Animals Autoimmune Diseases / drug therapy*, immunology, metabolism Blotting, Western Cell Proliferation Cytokines / metabolism Disease Models, Animal Female Flow Cytometry Hypoglycemic Agents / administration & dosage, therapeutic use Immunity, Cellular* Injections, Intraperitoneal Mice Mice, Inbred C57BL Ribonucleotides / administration & dosage*, therapeutic use T-Lymphocytes / immunology* Treatment Outcome Uvea / immunology, metabolism, pathology Uveitis / drug therapy*, immunology, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 0/Hypoglycemic Agents; 0/Ribonucleotides; 360-97-4/Aminoimidazole Carboxamide; F0X88YW0YK/AICA ribonucleotide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Positioning of electronic subretinal implants in blind retinitis pigmentosa patients through multimo...
Next Document: Responses of cells in the midbrain near-response area in monkeys with strabismus.