Document Detail


Amino acids supply in culture media is not a limiting factor in the matrix synthesis of engineered cartilage tissue.
MedLine Citation:
PMID:  17713744     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increased amino acid supplementation (0.5 x, 1.0 x, and 5.0 x recommended concentrations or additional proline) was hypothesized to increase the collagen content in engineered cartilage. No significant differences were found between groups in matrix content or dynamic modulus. Control constructs possessed the highest compressive Young's modulus on day 42. On day 42, compared to controls, decreased type II collagen was found with 0.5 x, 1.0 x, and 5.0 x supplementation and significantly increased DNA content found in 1.0 x and 5.0 x. No effects were observed on these measures with added proline. These results lead us to reject our hypothesis and indicate that the low collagen synthesis in engineered cartilage is not due to a limited supply of amino acids in media but may require a further stimulatory signal. The results of this study also highlight the impact that culture environment can play on the development of engineered cartilage.
Authors:
K W Ng; J G DeFrancis; L E Kugler; T-A N Kelly; M M Ho; C J O'Conor; G A Ateshian; C T Hung
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-08-24
Journal Detail:
Title:  Amino acids     Volume:  35     ISSN:  1438-2199     ISO Abbreviation:  Amino Acids     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-15     Completed Date:  2008-10-09     Revised Date:  2013-09-16    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  433-8     Citation Subset:  IM    
Affiliation:
Cellular Engineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / administration & dosage*
Animals
Cartilage / cytology,  metabolism*
Cattle
Cells, Cultured
Collagen Type II / biosynthesis
Culture Media / chemistry*
DNA / analysis
Dietary Supplements*
Tissue Engineering*
Grant Support
ID/Acronym/Agency:
AR 46568/AR/NIAMS NIH HHS; R01 AR046568/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Collagen Type II; 0/Culture Media; 9007-49-2/DNA
Comments/Corrections

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