Document Detail

Amino acids can induce nondisjunction during maturation of mammalian oocytes in vitro.
MedLine Citation:
PMID:  3989495     Owner:  NLM     Status:  MEDLINE    
Certain chromosomal disorders in mammalian embryos are traceable to meiotic errors during oocyte maturation. This report evaluates the influence of amino acids on meiotic maturation in vitro of oocytes from pigs, hamsters, and rats. The results indicate that maturing porcine oocytes respond not only to 1-glutamine (1-gln) but also to 1-isoleucine (1-ileu) in complex or chemically defined media by exhibiting significantly (P less than .05) increased incidences of nondisjunction when compared with oocytes in control medium. Nondisjunction was highly correlated (r = 0.981) with dose of 1-gln in porcine oocytes (incidences of maturing oocytes exhibiting nondisjunction were 19.3%, 39.7%, 41.5%, 66.2%, and 88.5% at 1-gln concentrations of 0 mM, 0.5 mM, 1.0 mM, 3.0 mM and 10.0 mM, respectively). Hamster oocytes also exhibited significantly (P less than .05) increased nondisjunction when cultured in medium containing 1-gln (52% of maturing cumulus-enclosed oocytes exhibited nondisjunction in medium with 3.0 mM 1-gln vs. 6.3% in control medium). Denuded hamster oocytes also responded to 1-gln (35% exhibited nondisjunction in 4.0 mM 1-gln, 8% in control medium). In contrast the incidence of nondisjunction in rat oocytes was not increased significantly by 1-gln over a concentration range of 0-12.0 mM. This study demonstrates that maturing oocytes respond to certain environmental conditions by undergoing chromosomally abnormal maturation. Specifically, amino acids can induce nondisjunction when present in elevated concentrations during oocyte maturation. The amino acid influence not only was dose dependent for porcine oocytes but also was species specific.(ABSTRACT TRUNCATED AT 250 WORDS)
R W McGaughey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  234     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1985 Apr 
Date Detail:
Created Date:  1985-06-03     Completed Date:  1985-06-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  161-5     Citation Subset:  IM    
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MeSH Terms
Amino Acids / pharmacology*
Nondisjunction, Genetic / drug effects*
Oocytes / physiology*
Grant Support
Reg. No./Substance:
0/Amino Acids

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