Document Detail


Amino acid uptake in plasma membrane vesicles isolated from proliferating tumor cells and tissues.
MedLine Citation:
PMID:  24194178     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The transport of L-alanine, a natural substrate of system A, across plasma membrane vesicle preparations has been studied in the early stages of rat DENA-PH hepato-carcinogenesis and in a very undifferentiated rat ascites hepatoma cell line (Yoshida AH-130) in the exponential and stationary phase of growth.Kinetic analyses indicated an increase of the Vmax value in DENA-PH-treated rats 30 h after partial hepatectomy as well as in exponential growing Yoshida ascites cells. In DENA-PH-treated rats the Km value was drastically reduced 7 and 60 days after surgery, when enzyme-altered hyperplastic and preneoplastic lesions were present in rat liver. Drastically reduced Km values were also found in Yoshida ascites cells.The results suggest that an altered alanine transporter might take place in liver plasma membranes from carcinogen-treated rats. This appears to occur also in an established tumor cell line, grown in vivo.
Authors:
M G Leonardi; R Comolli
Related Documents :
2559958 - Rat bioassay of wheat bran folate and effects of intestinal bacteria.
3500898 - Proliferative effects of urogastrone-egf on the intestinal epithelium.
23049818 - Dysregulation of micrornas in colonic field carcinogenesis: implications for screening.
3814618 - Impaired formylation and uptake of tetrahydrofolate by rat small gut following cobalami...
1268818 - Enhancing effect of allopurinol on the induction of bladder cancer in rats by n-[4-(5-n...
12868588 - A sulphur matrix complex, elastic fibril composed of a fine core of amorphous elastin a...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Amino acids     Volume:  1     ISSN:  0939-4451     ISO Abbreviation:  Amino Acids     Publication Date:  1991 Oct 
Date Detail:
Created Date:  2013-11-06     Completed Date:  2013-11-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  379-84     Citation Subset:  -    
Affiliation:
Dipartimento di Fisiologia e Biochimica Generali, Sezione Patologia Generale, Universita' di Milano, via Celoria 26, I-20133, Milano, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Preparation and analysis of a volatile derivative of cysteic acid.
Next Document:  The prognostic value of apoptotic and proliferative markers in breast cancer.