Document Detail

Amino acid uptake in plasma membrane vesicles isolated from proliferating tumor cells and tissues.
MedLine Citation:
PMID:  24194178     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The transport of L-alanine, a natural substrate of system A, across plasma membrane vesicle preparations has been studied in the early stages of rat DENA-PH hepato-carcinogenesis and in a very undifferentiated rat ascites hepatoma cell line (Yoshida AH-130) in the exponential and stationary phase of growth.Kinetic analyses indicated an increase of the Vmax value in DENA-PH-treated rats 30 h after partial hepatectomy as well as in exponential growing Yoshida ascites cells. In DENA-PH-treated rats the Km value was drastically reduced 7 and 60 days after surgery, when enzyme-altered hyperplastic and preneoplastic lesions were present in rat liver. Drastically reduced Km values were also found in Yoshida ascites cells.The results suggest that an altered alanine transporter might take place in liver plasma membranes from carcinogen-treated rats. This appears to occur also in an established tumor cell line, grown in vivo.
M G Leonardi; R Comolli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Amino acids     Volume:  1     ISSN:  0939-4451     ISO Abbreviation:  Amino Acids     Publication Date:  1991 Oct 
Date Detail:
Created Date:  2013-11-06     Completed Date:  2013-11-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  379-84     Citation Subset:  -    
Dipartimento di Fisiologia e Biochimica Generali, Sezione Patologia Generale, Universita' di Milano, via Celoria 26, I-20133, Milano, Italy.
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