| Amino acid supply and protein metabolism in Ehrlich ascites tumour cells. 1. Identification of limiting amino acids. | |
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MedLine Citation:
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PMID: 3622007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The uptake and intracellular accumulation of an amino acid mixture by incubated Ehrlich ascites tumour cells was studied. The composition of the amino acid mixture simulated that of mouse intraperitoneal fluid and amino acid uptake was studied over a range of concentrations between 0.0 (no added amino acids) and 10.0-times intraperitoneal concentrations. For most amino acids uptake into cells and intracellular accumulation occurred as concentrations were increased up to 6.0-times the intraperitoneal concentrations; further increases in external amino acid concentrations did not increase concomitantly with intracellular concentrations. These data, when analysed indicated a net protein synthetic rate of 20% d-1 and that the rate of protein synthesis may be limited by the availability of the amino acids lysine, threonine and methionine. |
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Authors:
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D L Doolan; L C Ward |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytobios Volume: 51 ISSN: 0011-4529 ISO Abbreviation: Cytobios Publication Date: 1987 |
Date Detail:
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Created Date: 1987-10-16 Completed Date: 1987-10-16 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0207227 Medline TA: Cytobios Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 7-23 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Animals Biological Transport Carcinoma, Ehrlich Tumor / metabolism*, pathology Cell Survival Kinetics Mice Neoplasm Proteins / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Neoplasm Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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