Document Detail

Amino acid supply and protein metabolism in Ehrlich ascites tumour cells. 1. Identification of limiting amino acids.
MedLine Citation:
PMID:  3622007     Owner:  NLM     Status:  MEDLINE    
The uptake and intracellular accumulation of an amino acid mixture by incubated Ehrlich ascites tumour cells was studied. The composition of the amino acid mixture simulated that of mouse intraperitoneal fluid and amino acid uptake was studied over a range of concentrations between 0.0 (no added amino acids) and 10.0-times intraperitoneal concentrations. For most amino acids uptake into cells and intracellular accumulation occurred as concentrations were increased up to 6.0-times the intraperitoneal concentrations; further increases in external amino acid concentrations did not increase concomitantly with intracellular concentrations. These data, when analysed indicated a net protein synthetic rate of 20% d-1 and that the rate of protein synthesis may be limited by the availability of the amino acids lysine, threonine and methionine.
D L Doolan; L C Ward
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytobios     Volume:  51     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  1987  
Date Detail:
Created Date:  1987-10-16     Completed Date:  1987-10-16     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  7-23     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism*
Biological Transport
Carcinoma, Ehrlich Tumor / metabolism*,  pathology
Cell Survival
Neoplasm Proteins / biosynthesis*
Reg. No./Substance:
0/Amino Acids; 0/Neoplasm Proteins

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