| Amino acid substitutions in naturally occurring variants of ail result in altered invasion activity. | |
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MedLine Citation:
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PMID: 1370953 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Yersinia enterocolitica is the causative agent of a variety of gastrointestinal syndromes ranging from acute enteritis to mesenteric lymphadenitis. In addition, systemic infections resulting in high mortality rates can occur in elderly and immunocompromised patients. More than 50 serotypes of Y. enterocolitica have been identified, but only a few of them commonly cause disease in otherwise healthy hosts. Those serotypes that cause disease have been divided into two groups, American and non-American, based on their geographical distributions, biotypes, and pathogenicity. We have been studying two genes, inv and ail, from Y. enterocolitica that confer in tissue culture assays an invasive phenotype that strongly correlates with virulence. Some differences between the American and non-American serotypes at the ail locus were noted previously and have been investigated further in this report. The ail locus was cloned from seven Y. enterocolitica strains (seven different serotypes). Although the different clones produced similar amounts of Ail, the product of the ail gene from non-American serotypes (AilNA) was less able to promote invasion by Escherichia coli than was the product of the ail gene from American serotypes (AilA). This difference is probably due to one or more of the eight amino acid changes found in the derived amino acid sequence for the mature form of AilNA compared with that of AilA. Seven of these changes are predicted to be in cell surface domains of the protein (a model for the proposed folding of Ail within the outer membrane is presented). These results are discussed in relation to the growing family of outer membrane proteins, which includes Lom from bacteriophage lambda, PagC from salmonella typhimurium, and OmpX from Enterobacter cloacae. |
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Authors:
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K B Beer; V L Miller |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 174 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1992 Feb |
Date Detail:
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Created Date: 1992-03-10 Completed Date: 1992-03-10 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1360-9 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024-1489. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / chemistry Animals Bacterial Outer Membrane Proteins / biosynthesis, chemistry*, genetics, immunology Base Sequence Blotting, Southern Blotting, Western CHO Cells Cell Line Cloning, Molecular Cricetinae Epitopes Humans Molecular Sequence Data Phenotype Protein Conformation Restriction Mapping Serotyping Virulence Yersinia enterocolitica / chemistry, classification, genetics, pathogenicity* |
| Grant Support | |
ID/Acronym/Agency:
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AI27342/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ail protein, Yersinia enterocolitica; 0/Amino Acids; 0/Bacterial Outer Membrane Proteins; 0/Epitopes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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