| Amino acid substitutions at position 95 in GyrA can add fluoroquinolone resistance to Mycobacterium leprae. | |
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MedLine Citation:
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PMID: 22106221 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Amino acid substitutions at position 89 or 91 in GyrA of fluoroquinolone-resistant Mycobacterium leprae clinical isolates have been reported. In contrast, those at position 94 in M. tuberculosis, equivalent to position 95 in M. leprae, have been identified most frequently. To verify the possible contribution of amino acid substitutions at position 95 in M. leprae to fluoroquinolone resistance, we conducted an in vitro assay using wild-type and mutant recombinant DNA gyrases. Fluoroquinolone-mediated supercoiling activity inhibition assay and DNA cleavage assay revealed the potent contribution of an amino acid substitution of Asp to Gly or Asn at position 95 to fluoroquinolone resistance. These results suggested the possible future emergence of quinolone-resistant M. leprae isolates with these amino acid substitutions and the usefulness of detecting these mutations for the rapid identification of fluoroquinolone resistance in leprosy. |
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Authors:
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Kazumasa Yokoyama; Hyun Kim; Tetsu Mukai; Masanori Matsuoka; Chie Nakajima; Yasuhiko Suzuki |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-11-21 |
Journal Detail:
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Title: Antimicrobial agents and chemotherapy Volume: 56 ISSN: 1098-6596 ISO Abbreviation: Antimicrob. Agents Chemother. Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-23 Completed Date: 2012-05-25 Revised Date: 2012-09-25 |
Medline Journal Info:
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Nlm Unique ID: 0315061 Medline TA: Antimicrob Agents Chemother Country: United States |
Other Details:
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Languages: eng Pagination: 697-702 Citation Subset: IM |
Affiliation:
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Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Substitution* Anti-Bacterial Agents / pharmacology* Base Sequence DNA Gyrase / chemistry, genetics* Drug Resistance, Bacterial / genetics* Fluoroquinolones / pharmacology* Humans Microbial Sensitivity Tests Molecular Sequence Data Mycobacterium leprae / drug effects*, enzymology, genetics |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Fluoroquinolones; EC 5.99.1.-/DNA Gyrase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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