Document Detail


Amino acid substitutions at position 95 in GyrA can add fluoroquinolone resistance to Mycobacterium leprae.
MedLine Citation:
PMID:  22106221     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amino acid substitutions at position 89 or 91 in GyrA of fluoroquinolone-resistant Mycobacterium leprae clinical isolates have been reported. In contrast, those at position 94 in M. tuberculosis, equivalent to position 95 in M. leprae, have been identified most frequently. To verify the possible contribution of amino acid substitutions at position 95 in M. leprae to fluoroquinolone resistance, we conducted an in vitro assay using wild-type and mutant recombinant DNA gyrases. Fluoroquinolone-mediated supercoiling activity inhibition assay and DNA cleavage assay revealed the potent contribution of an amino acid substitution of Asp to Gly or Asn at position 95 to fluoroquinolone resistance. These results suggested the possible future emergence of quinolone-resistant M. leprae isolates with these amino acid substitutions and the usefulness of detecting these mutations for the rapid identification of fluoroquinolone resistance in leprosy.
Authors:
Kazumasa Yokoyama; Hyun Kim; Tetsu Mukai; Masanori Matsuoka; Chie Nakajima; Yasuhiko Suzuki
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-21
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  56     ISSN:  1098-6596     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-23     Completed Date:  2012-05-25     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  United States    
Other Details:
Languages:  eng     Pagination:  697-702     Citation Subset:  IM    
Affiliation:
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Substitution*
Anti-Bacterial Agents / pharmacology*
Base Sequence
DNA Gyrase / chemistry,  genetics*
Drug Resistance, Bacterial / genetics*
Fluoroquinolones / pharmacology*
Humans
Microbial Sensitivity Tests
Molecular Sequence Data
Mycobacterium leprae / drug effects*,  enzymology,  genetics
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Fluoroquinolones; EC 5.99.1.-/DNA Gyrase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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