| Amino acid substitutions at position 95 in GyrA can add fluoroquinolone resistance to Mycobacterium leprae. | |
| | |
MedLine Citation:
|
PMID: 22106221 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Amino acid substitutions in GyrA of fluoroquinolone-resistant Mycobacterium leprae clinical isolates have been reported at position 89 or 91. In contrast, those at position 94 in M. tuberculosis, equivalent to position 95 in M. leprae, have been identified most frequently. To verify the possible contribution of amino acid substitutions at position 95 in M. leprae to fluoroquinolone resistance, we conducted an in vitro assay using wild-type and mutant recombinant DNA gyrases. Fluoroquinolone-mediated supercoiling activity inhibition assay and DNA cleavage assay revealed the potent contribution of amino acid substitution, Asp to Gly or Asn at position 95, to fluoroquinolone resistance. These results suggested the possible future emergence of quinolone-resistant M. leprae with these amino acid substitutions and the usefulness of detecting these mutations for the rapid identification of fluoroquinolone resistance in leprosy. |
| | |
Authors:
|
Kazumasa Yokoyama; Hyun Kim; Tetsu Mukai; Masanori Matsuoka; Chie Nakajima; Yasuhiko Suzuki |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-21 |
Journal Detail:
|
Title: Antimicrobial agents and chemotherapy Volume: - ISSN: 1098-6596 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-22 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0315061 Medline TA: Antimicrob Agents Chemother Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita 20, Nishi 10, Kita-ku, Sapporo 001-0020, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Virulence factors and TEM- type ?-lactamases produced by two isolates of an epidemic Klebsiella pneu...
Next Document: Changes in ciprofloxacin resistance levels in Enterobacter aerogenes associated to variable expressi...