| Amino acid substitution converts WEREWOLF function from an activator to a repressor of Arabidopsis non-hair cell development. | |
| | |
MedLine Citation:
|
PMID: 22195575 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Root hair cell or non-hair cell fate determination in Arabidopsis thaliana root epidermis is model system for plant cell development. Two types of MYB transcription factors, the R2R3-type MYB, WEREWOLF (WER), and an R3-type MYB, CAPRICE (CPC), are involved in this cell fate determination process. To study the molecular basis of this process, we analyzed the functional relationship of WER and CPC. WER-CPC chimeric constructs were made from WER where all or parts of the MYB R3 region were replaced with the corresponding regions from CPC R3, and the constructs were introduced into the cpc-2 mutant. Although, the WER gene did not rescue the cpc-2 mutant 'small number of root hairs' phenotype, the WER-CPC chimera with two amino acids substitution (WC6) completely rescued the cpc-2 mutant phenotype. Furthermore, the WER-CPC chimera with 37 amino acids substitution (WC5) excessively rescued the cpc-2 mutant and induced 2.5 times more root hairs than wild-type. Consistent with this phenotype, GL2 gene expression was strongly reduced in WC5 in a cpc-2 background. Our results suggest that swapping at least two amino acids is sufficient to convert WER to CPC function. Therefore, these key residues may have strongly contributed to the selection of these important functions over evolution. |
| | |
Authors:
|
Rumi Tominaga-Wada; Yuka Nukumizu; Takuji Wada |
Related Documents
:
|
7112085 - The effect of megadose ascorbic acid ingestion on the absorption and retention of vitam... 2665185 - Comparative assessment of the toxicology of vitamin a and retinoids in man. 9578515 - Evidence for modulation of osteocalcin containing gamma-carboxyglutamic acid residues s... 7472665 - Methylmalonic acid inhibits respiration in rat liver mitochondria. 17177375 - Chiral photocages based on phthalimide photochemistry. 16939355 - Color properties of four cyanidin-pyruvic acid adducts. |
Publication Detail:
|
Type: Journal Article Date: 2011-11-06 |
Journal Detail:
|
Title: Plant science : an international journal of experimental plant biology Volume: 183 ISSN: 1873-2259 ISO Abbreviation: Plant Sci. Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2011-12-26 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9882015 Medline TA: Plant Sci Country: Ireland |
Other Details:
|
Languages: eng Pagination: 37-42 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Interdisciplinary Research Organization, University of Miyazaki, 1-1, Gakuen Kibanadai-Nishi, Miyazaki 889-2192, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Dehydrin metabolism is altered during seed osmopriming and subsequent germination under chilling and...
Next Document: Involvement of the leaf antioxidant system in the response to soil flooding in two Trifolium genotyp...