Document Detail


Amino acid sequences of ferredoxins from Atropa belladonna and Hyoscyamus niger: their similarities to those in other tropane-alkaloid-containing plants.
MedLine Citation:
PMID:  16079510     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The complete amino acid sequences of [2Fe-2S] ferredoxin from Atropa belladonna and Hyoscyamus niger have been determined by automated Edman degradation of the entire S-carboxymethylcysteinyl proteins and of the peptides obtained by enzymatic digestion. These two ferredoxins exhibited 1-8 differences in their amino acid sequences compared to those of other tropane-alkaloid-containing plants (Scopolia japonica, Datura stramonium, D. metel, and D. arborea), and only 1 or 4 differences compared to S. japonica and D. arborea. In contrast, 9-23 differences were observed among the other solanaceous ferredoxins. This suggests that tropane-alkaloid-containing plants are closely related taxonomically.
Authors:
Yoshiki Mino; Mayumi Yukita; Nobuhiro Hiratsuka; Hiroyuki Wariishi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  28     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-04     Completed Date:  2005-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1535-8     Citation Subset:  IM    
Affiliation:
Department of Environment Analysis, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. mino@gly.oups.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Atropa belladonna / chemistry*,  classification
Ferredoxins / chemistry*,  classification,  isolation & purification
Hyoscyamus / chemistry*,  classification
Molecular Sequence Data
Phylogeny
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/Ferredoxins

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