Document Detail


Amino-acid sequence of the short subfragment-2 in adult chicken skeletal muscle myosin.
MedLine Citation:
PMID:  2775482     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The amino-acid sequence of a short subfragment-2 in the amino-terminal portion of subfragment-2 (S-2) derived from adult chicken skeletal muscle myosin was completely determined. Peptides cleaved by cyanogen bromide and by lysyl endopeptidase of S-carboxymethylated S-2, and hydrolytic peptides obtained with trypsin or dilute acetic acid of larger CNBr fragments were isolated and sequenced. This region was composed of 257 amino-acid residues, and hydrophobic and charged residue repeat units were found highly conserved and with a periodicity in 7 or 28 residues. This sequence of the short S-2 fragment of chicken skeletal muscle myosin was compared with the sequence of chicken and rat embryonic skeletal muscle myosins, rabbit skeletal and rabbit cardiac muscle myosin (alpha-myosin heavy chain), and 95.3%, 86.8%, 89.9% and 94.2% sequence identities were observed, respectively.
Authors:
B Watanabe
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological chemistry Hoppe-Seyler     Volume:  370     ISSN:  0177-3593     ISO Abbreviation:  Biol. Chem. Hoppe-Seyler     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-10-23     Completed Date:  1989-10-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8503054     Medline TA:  Biol Chem Hoppe Seyler     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  549-58     Citation Subset:  IM    
Affiliation:
School of Allied Medical Sciences, Nagasaki University.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis
Animals
Chickens
Cyanogen Bromide
Molecular Sequence Data
Muscles
Myosin Subfragments
Myosins* / isolation & purification
Peptide Fragments* / isolation & purification
Sequence Homology, Nucleic Acid
Serine Endopeptidases
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Myosin Subfragments; 0/Peptide Fragments; 506-68-3/Cyanogen Bromide; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.50/lysyl endopeptidase; EC 3.6.4.1/Myosins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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