| Amino acid sequence of a Crotalus atrox venom metalloproteinase which cleaves type IV collagen and gelatin. | |
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MedLine Citation:
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PMID: 2745407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hemorrhagic toxin Ht-d from venom of the Western diamondback rattlesnake is a metalloproteinase with a molecular weight of 23,234. Peptides were obtained from enzymatic and chemical digestions, separated by reverse-phase chromatography, and sequenced in a gas-phase sequenator. The sequence showed a putative zinc binding site similar to that of thermolysin and other metalloproteinases but no overall significant similarity to the sequences of other metalloproteinases and may represent a new subfamily of metalloproteinases. Ht-d was shown to degrade type IV collagen and gelatin types I, III, and V but not interstitial collagens. The digestion of type IV collagen and other basement membrane proteins may allow this proteinase to disrupt capillary membranes causing hemorrhage in surrounding tissues. |
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Authors:
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J D Shannon; E N Baramova; J B Bjarnason; J W Fox |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 264 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1989 Jul |
Date Detail:
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Created Date: 1989-08-14 Completed Date: 1989-08-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 11575-83 Citation Subset: IM |
Affiliation:
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Department of Microbiology, School of Medicine, University of Virginia, Charlottesville 22908. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / analysis* Animals Binding Sites Chromatography, High Pressure Liquid Collagen / metabolism* Crotalid Venoms / metabolism* Electrophoresis, Polyacrylamide Gel Gelatin / metabolism* Hydrolysis Metalloendopeptidases / antagonists & inhibitors, metabolism* Molecular Sequence Data Rats Substrate Specificity Zinc / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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GM31289/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Crotalid Venoms; 7440-66-6/Zinc; 9000-70-8/Gelatin; 9007-34-5/Collagen; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.1/atrolysin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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