Document Detail

Amino acid sequence of a Crotalus atrox venom metalloproteinase which cleaves type IV collagen and gelatin.
MedLine Citation:
PMID:  2745407     Owner:  NLM     Status:  MEDLINE    
The hemorrhagic toxin Ht-d from venom of the Western diamondback rattlesnake is a metalloproteinase with a molecular weight of 23,234. Peptides were obtained from enzymatic and chemical digestions, separated by reverse-phase chromatography, and sequenced in a gas-phase sequenator. The sequence showed a putative zinc binding site similar to that of thermolysin and other metalloproteinases but no overall significant similarity to the sequences of other metalloproteinases and may represent a new subfamily of metalloproteinases. Ht-d was shown to degrade type IV collagen and gelatin types I, III, and V but not interstitial collagens. The digestion of type IV collagen and other basement membrane proteins may allow this proteinase to disrupt capillary membranes causing hemorrhage in surrounding tissues.
J D Shannon; E N Baramova; J B Bjarnason; J W Fox
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  264     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1989 Jul 
Date Detail:
Created Date:  1989-08-14     Completed Date:  1989-08-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  11575-83     Citation Subset:  IM    
Department of Microbiology, School of Medicine, University of Virginia, Charlottesville 22908.
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MeSH Terms
Amino Acid Sequence
Amino Acids / analysis*
Binding Sites
Chromatography, High Pressure Liquid
Collagen / metabolism*
Crotalid Venoms / metabolism*
Electrophoresis, Polyacrylamide Gel
Gelatin / metabolism*
Metalloendopeptidases / antagonists & inhibitors,  metabolism*
Molecular Sequence Data
Substrate Specificity
Zinc / metabolism
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Crotalid Venoms; 7440-66-6/Zinc; 9000-70-8/Gelatin; 9007-34-5/Collagen; EC 3.4.24.-/Metalloendopeptidases; EC A

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