| Amino acid residues essential for catalysis by peptidyl dipeptidase-4 from Pseudomonas maltophilia. | |
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MedLine Citation:
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PMID: 2563225 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To assess residues essential for catalysis by prokaryotic peptidyl dipeptidase-4, the enzyme was subjected to chemical modification by a series of reagents. Treatment with either tetranitromethane or N-acetylimidazole abolished catalytic activity. Hydroxylamine reversed inactivation by acetylimidazole only. Thus, an essential tyrosine is indicated. Enzymatic activity also was quenched by either trinitrobenzenesulfonic acid or diethyl pyrocarbonate. Inactivation by these reagents was not reversed by hydroxylamine. These data suggest an essential lysine. The competitive inhibitor Phe-Arg protected partially against inactivation by tetranitromethane, and fully against inactivation by N-acetylimidazole. The substrate Hip-Phe-Arg protected against inactivation by trinitrobenzenesulfonic acid and diethyl pyrocarbonate. Thus, both tyrosine and lysine are located at the catalytic site. |
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Authors:
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J J Lanzillo; Y Dasarathy; B L Fanburg |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 158 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1989 Jan |
Date Detail:
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Created Date: 1989-02-17 Completed Date: 1989-02-17 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 45-51 Citation Subset: IM |
Affiliation:
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New England Medical Center Hospital, Department of Medicine, Boston, MA 02111. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Antigens, CD26 Catalysis Diethyl Pyrocarbonate / pharmacology Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / antagonists & inhibitors, metabolism* Kinetics Pseudomonas / enzymology* Substrate Specificity Tetranitromethane / pharmacology Trinitrobenzenesulfonic Acid / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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HL07053/HL/NHLBI NIH HHS; HL14456/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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1609-47-8/Diethyl Pyrocarbonate; 2508-19-2/Trinitrobenzenesulfonic Acid; 509-14-8/Tetranitromethane; EC 3.4.14.-/Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; EC 3.4.14.5/Antigens, CD26 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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