Document Detail

Amino acid residues essential for catalysis by peptidyl dipeptidase-4 from Pseudomonas maltophilia.
MedLine Citation:
PMID:  2563225     Owner:  NLM     Status:  MEDLINE    
To assess residues essential for catalysis by prokaryotic peptidyl dipeptidase-4, the enzyme was subjected to chemical modification by a series of reagents. Treatment with either tetranitromethane or N-acetylimidazole abolished catalytic activity. Hydroxylamine reversed inactivation by acetylimidazole only. Thus, an essential tyrosine is indicated. Enzymatic activity also was quenched by either trinitrobenzenesulfonic acid or diethyl pyrocarbonate. Inactivation by these reagents was not reversed by hydroxylamine. These data suggest an essential lysine. The competitive inhibitor Phe-Arg protected partially against inactivation by tetranitromethane, and fully against inactivation by N-acetylimidazole. The substrate Hip-Phe-Arg protected against inactivation by trinitrobenzenesulfonic acid and diethyl pyrocarbonate. Thus, both tyrosine and lysine are located at the catalytic site.
J J Lanzillo; Y Dasarathy; B L Fanburg
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  158     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-02-17     Completed Date:  1989-02-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  45-51     Citation Subset:  IM    
New England Medical Center Hospital, Department of Medicine, Boston, MA 02111.
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MeSH Terms
Amino Acid Sequence
Antigens, CD26
Diethyl Pyrocarbonate / pharmacology
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / antagonists & inhibitors,  metabolism*
Pseudomonas / enzymology*
Substrate Specificity
Tetranitromethane / pharmacology
Trinitrobenzenesulfonic Acid / pharmacology
Grant Support
Reg. No./Substance:
1609-47-8/Diethyl Pyrocarbonate; 2508-19-2/Trinitrobenzenesulfonic Acid; 509-14-8/Tetranitromethane; EC 3.4.14.-/Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; EC, CD26

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