Document Detail

Amino acid requirements in humans: with a special emphasis on the metabolic availability of amino acids.
MedLine Citation:
PMID:  19156481     Owner:  NLM     Status:  MEDLINE    
Due to advances made in the development of stable isotope based carbon oxidation methods, the determination of amino acid requirements in humans has been an active area of research for the past 2 decades. The indicator amino acid oxidation (IAAO) method developed in our laboratory for humans has been systematically applied to determine almost all indispensable amino acid requirements in adult humans. Nutritional application of experimentally derived amino acid requirement estimates depends upon the capacity of food proteins to meet the amino acid requirements in humans. Therefore, there is a need to know the proportion of dietary amino acids which are bioavailable, or metabolically available to the body for protein synthesis following digestion and absorption. Although this concept is widely applied in animal nutrition, it has not been applied to human nutrition due to lack of data. We developed a new in vivo method in growing pigs to identify the metabolic availability of amino acids in foods using the IAAO concept. This metabolic availability method has recently been adapted for use in humans. As this newly developed IAAO based method to determine metabolic availability of amino acids in foods is suitable for rapid and routine analysis in humans, it is a major step forward in defining the protein quality of food sources and integrating amino acid requirement data with dietary amino acid availability of foods.
Rajavel Elango; Ronald O Ball; Paul B Pencharz
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2009-01-21
Journal Detail:
Title:  Amino acids     Volume:  37     ISSN:  1438-2199     ISO Abbreviation:  Amino Acids     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-04     Completed Date:  2009-07-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  19-27     Citation Subset:  IM    
Research Institute, The Hospital for Sick Children, Toronto, ON, Canada.
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MeSH Terms
Amino Acids / metabolism*
Biological Availability
Carbon Isotopes / metabolism
Dietary Proteins / metabolism*
Metabolic Networks and Pathways*
Nutrition Assessment
Nutritional Requirements*
Swine / metabolism
Reg. No./Substance:
0/Amino Acids; 0/Carbon Isotopes; 0/Dietary Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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