Document Detail


Amino acid regions of family 45 endoglucanases involved in cotton defibrillation and in resistance to anionic surfactants and oxidizing agents.
MedLine Citation:
PMID:  17031034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the detergent industry, fungal endoglucanases are used to release microfibrils from the surfaces of dyed cellulosic fabrics to enhance color brightness. Family 45 endoglucanase (glycoside hydrolase family 45, GH45) EGL3 from Humicola grisea is more resistant to anionic surfactants and oxidizing agents than family 45 endoglucanase RCE1 from Rhizopus oryzae, while in the present study, a catalytic domain of RCE1 had higher defibrillation activity on dyed cotton fabrics than did that of EGL3. To identify the amino acid regions involved in these properties, we compared the characteristics of RCE1, EGL3, and three chimeric endoglucanases, in which each of the three regions of the catalytic domain of EGL3 was replaced by the corresponding region of the catalytic domain of RCE1. Amino acids in the N-terminal region were involved in resistance to anionic surfactants and oxidizing agents. Furthermore, amino acids in the region adjacent to the N-terminal region were involved in releasing microfibrils and in binding to dyed cotton fabrics, indicating that the binding of the amino acids in this region might be important in the release of microfibrils from dyed cotton fabrics.
Authors:
Atsushi Shimonaka; Koichiro Murashima; Jinichiro Koga; Yuko Baba; Tomoko Nishimura; Hidetoshi Kubota; Toshiaki Kono
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Publication Detail:
Type:  Journal Article     Date:  2006-10-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  70     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-23     Completed Date:  2007-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2460-6     Citation Subset:  IM    
Affiliation:
Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd, Japan
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MeSH Terms
Descriptor/Qualifier:
Amino Acids
Anions
Cellulases / chemistry*,  metabolism
Coloring Agents
Cotton Fiber / methods*
Detergents / chemistry*
Oxidants
Surface-Active Agents
Textile Industry / methods
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Anions; 0/Coloring Agents; 0/Detergents; 0/Oxidants; 0/Surface-Active Agents; EC 3.2.1.-/Cellulases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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