Document Detail

Amino acid regions of family 45 endoglucanases involved in cotton defibrillation and in resistance to anionic surfactants and oxidizing agents.
MedLine Citation:
PMID:  17031034     Owner:  NLM     Status:  MEDLINE    
In the detergent industry, fungal endoglucanases are used to release microfibrils from the surfaces of dyed cellulosic fabrics to enhance color brightness. Family 45 endoglucanase (glycoside hydrolase family 45, GH45) EGL3 from Humicola grisea is more resistant to anionic surfactants and oxidizing agents than family 45 endoglucanase RCE1 from Rhizopus oryzae, while in the present study, a catalytic domain of RCE1 had higher defibrillation activity on dyed cotton fabrics than did that of EGL3. To identify the amino acid regions involved in these properties, we compared the characteristics of RCE1, EGL3, and three chimeric endoglucanases, in which each of the three regions of the catalytic domain of EGL3 was replaced by the corresponding region of the catalytic domain of RCE1. Amino acids in the N-terminal region were involved in resistance to anionic surfactants and oxidizing agents. Furthermore, amino acids in the region adjacent to the N-terminal region were involved in releasing microfibrils and in binding to dyed cotton fabrics, indicating that the binding of the amino acids in this region might be important in the release of microfibrils from dyed cotton fabrics.
Atsushi Shimonaka; Koichiro Murashima; Jinichiro Koga; Yuko Baba; Tomoko Nishimura; Hidetoshi Kubota; Toshiaki Kono
Related Documents :
8980134 - Cloning and functional expression of a novel isoform of romk inwardly rectifying atp-de...
23772104 - Simultaneous determination of 5 phenolic acids in fried fructus xanthii from different ...
2414914 - Amino- and carboxyl-terminal sequence of moloney murine leukemia virus reverse transcri...
10462474 - Molecular cloning and sequence analysis of a cdna for factor v activating enzyme.
16839154 - Phosphine-free cross-coupling reaction of arylboronic acids with carboxylic anhydrides ...
12495914 - Primary cell wall composition of bryophytes and charophytes.
Publication Detail:
Type:  Journal Article     Date:  2006-10-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  70     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-23     Completed Date:  2007-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2460-6     Citation Subset:  IM    
Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd, Japan
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids
Cellulases / chemistry*,  metabolism
Coloring Agents
Cotton Fiber / methods*
Detergents / chemistry*
Surface-Active Agents
Textile Industry / methods
Reg. No./Substance:
0/Amino Acids; 0/Anions; 0/Coloring Agents; 0/Detergents; 0/Oxidants; 0/Surface-Active Agents; EC 3.2.1.-/Cellulases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Removal of p-alkylphenols from aqueous solutions by combined use of mushroom tyrosinase and chitosan...
Next Document:  Dietary iron deficiency decreases serum osteocalcin concentration and bone mineral density in rats.