Document Detail


Amino acid odorants stimulate microvillar sensory neurons.
MedLine Citation:
PMID:  11923189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The olfactory epithelium (OE) of zebrafish is populated with ciliated and microvillar olfactory sensory neurons (OSNs). Whether distinct classes of odorants specifically activate either of these unique populations of OSNs is unknown. Previously we demonstrated that zebrafish OSNs could be labeled in an activity-dependent fashion by amino acid but not bile acid odorants. To determine which sensory neuron type was stimulated by amino acid odorants, we labeled OSNs using the ion channel permeant probe agmatine (AGB) and analyzed its distribution with conventional light- and electron-microscope immunocytochemical techniques. Approximately 7% of the sensory epithelium was labeled by AGB exposure alone. Following stimulation with one of the eight amino acids tested, the proportion of labeled epithelium increased from 9% for histidine to 19% for alanine; amino acid stimulated increases in labeling of 2-12% over control labeling. Only histidine failed to stimulate a significant increase in the proportion of labeled OSNs compared to control preparations. Most amino acid sensitive OSNs were located superficially in the epithelium and immuno-electron microscopy demonstrated that the labeled OSNs were predominantly microvillar. Large numbers of nanogold particles (20-60 per 1.5 microm(2)) were associated with microvillar olfactory sensory neurons (MSNs), while few such particles (<15 per 1.5 microm(2)) were observed over ciliated olfactory sensory neurons (CSNs), supporting cells (SCs) and areas without tissue, such as the lumen above the OE. Collectively, these findings indicate that microvillar sensory neurons are capable of detecting amino acid odorants.
Authors:
David L Lipschitz; William C Michel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chemical senses     Volume:  27     ISSN:  0379-864X     ISO Abbreviation:  Chem. Senses     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-29     Completed Date:  2002-09-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8217190     Medline TA:  Chem Senses     Country:  England    
Other Details:
Languages:  eng     Pagination:  277-86     Citation Subset:  IM    
Affiliation:
University of Utah School of Medicine, Department of Physiology, Salt Lake City, UT 84108-1297, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids*
Animals
Immunohistochemistry
Microscopy, Electron
Neurons / physiology*,  ultrastructure
Odors*
Zebrafish / physiology*
Grant Support
ID/Acronym/Agency:
DC 01418/DC/NIDCD NIH HHS; NS 07938/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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