Document Detail

Amino acid metabolism in leg muscle after an endotoxin injection in healthy volunteers.
MedLine Citation:
PMID:  15367399     Owner:  NLM     Status:  MEDLINE    
Decreased plasma amino acid concentrations and increased net release of amino acids from skeletal muscle, especially for glutamine, are common features in critically ill patients. A low dose of endotoxin administered to healthy volunteers was used as a human model for the initial phase of sepsis to study the early metabolic response to sepsis. Six healthy male volunteers were studied in the postabsorptive state. Blood samples from the forearm artery and femoral vein were taken during 4 h before and 4 h after an intravenous endotoxin injection (4 ng/kg body wt). In addition, muscle biopsies from the leg muscle were taken. Plasma concentration of the total sum of amino acids decreased by 19% (P = 0.001) and of glutamine by 25% (P = 0.004) the 3rd h after endotoxin administration. At the same time, muscle concentrations of the sum of amino acids and glutamine decreased by 11% (P = 0.05) and 9% (P = 0.09), respectively. In parallel, the efflux from the leg increased by 35% (P = 0.004) for the total sum of amino acids and by 43% (P = 0.05) for glutamine. In conclusion, intravenous endotoxin administration to healthy volunteers, used as a model for the initial phase of sepsis, resulted in a decrease in plasma amino acid concentrations. At the same time, amino acid concentrations in muscle tissue decreased, whereas the efflux of amino acids from leg skeletal muscle increased.
Rokhsareh F Vesali; Maria Klaude; Olav Rooyackers; Jan Wernerman
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-09-14
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  288     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-07     Completed Date:  2005-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E360-4     Citation Subset:  IM    
Department of Anesthesiology & Intensive Care, Karolinska Institutet, KFC Novum, Karolinska University Hospital, S-141 86 Huddinge, Sweden.
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MeSH Terms
Amino Acids / blood,  metabolism*
Endotoxins / administration & dosage*
Injections, Intravenous
Metabolic Clearance Rate
Muscle, Skeletal / blood supply,  metabolism*
Reference Values
Sepsis / chemically induced,  metabolism*
Thigh / physiopathology
Reg. No./Substance:
0/Amino Acids; 0/Endotoxins; 67924-63-4/endotoxin, Escherichia coli

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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