| Amino acid metabolism in isolated perfused rat liver. | |
| | |
MedLine Citation:
|
PMID: 2359297 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Conflicting evidence concerning hepatic amino acid (AA) metabolism in the isolated perfused rat liver (IPRL) led us to investigate the response of IPRL using perfusates with various AA contents. Perfusion (n = 4) with whole rat blood diluted in Krebs buffer (1:3, v/v) led to acute proteolysis on account of AA deprivation, as shown by the large release of AA (approximately 1400 mumoles in 120 min), especially branched-chain AA (BCAA) (e.g., Leu, 35.4 +/- 10.4 nmole.min-1.g-1 the first hour, 34.3 +/- 5.5 nmole.min-1.g-1 the second hour). In a first attempt to prevent proteolysis, livers (n = 4) were perfused with the previous medium supplemented with AA known for their antiproteolytic activity, at twice their physiological concentrations. Results during the first hour showed uptake of several AA (mainly alanine, glutamine, and proline), reduced release of BCAA (leucine, 12.5 +/- 6.3 nmole.min-1.g-1), and an increase in glucose and urea production. However, during the second hour, because of the use of a recirculating system, progressive AA depletion induced a reappearance of proteolysis. A two-step AA loading technique, i.e., the addition of antiproteolytic AA at the beginning of the perfusion and the addition of a balanced AA mixture at 60 min caused a further decrease in proteolysis during the 2 hr of perfusion (n = 6). Under these conditions, most AA were taken up by the liver with uptake values comparable to those observed in vivo. |
| | |
Authors:
|
J P De Bandt; L Cynober; F Ballet; C Coudray-Lucas; C Rey; J Giboudeau |
Related Documents
:
|
21783527 - Influence of age on noise- and styrene-induced hearing loss in the long-evans rat. 4072247 - Hepatic uptake and metabolism of pentacaine: a study with microsomes, hepatocytes and p... 7957447 - Kidney and liver contributions to salicylate metabolism in rats. 1277187 - Folate deficiency in the livers of diethylnitrosaminetreated rats. 4052067 - Inhibition of liver lipase in vivo leads to induction of de novo cholesterol synthesis ... 10203237 - Sex-specific expression of n-methyl d-aspartate receptor (nmdar) in the preoptic area o... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of surgical research Volume: 49 ISSN: 0022-4804 ISO Abbreviation: J. Surg. Res. Publication Date: 1990 Jul |
Date Detail:
|
Created Date: 1990-08-01 Completed Date: 1990-08-01 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 8-13 Citation Subset: IM |
Affiliation:
|
Laboratoire de Biochimie A, Hôpital St. Antoine, Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
metabolism* Animals Culture Techniques Liver / metabolism* Male Perfusion / methods Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Glucose uptake and amino acid metabolism in perfused hearts from tumor-bearing rats.
Next Document: Regulation of proliferation of peritoneal tissue repair cells by peritoneal macrophages.