Document Detail


Amino acid derivatives from Lucilia sericata excretions/secretions may contribute to the beneficial effects of maggot therapy via increased angiogenesis.
MedLine Citation:
PMID:  19799603     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Maggot therapy, utilizing the larvae of Lucilia sericata, has been reported to reduce the bacterial load within wounds and also to enhance wound healing. Maggot excretions/secretions (ES) have been shown to have a role in the success of maggot therapy. While the protein content of ES has been investigated, to date little research has focused on the small metabolites present in ES and their potential contribution to the therapy. Study of the molecular composition of the secretions and the potential bioactivities present will allow for a more detailed evaluation of the efficacy of maggot therapy. OBJECTIVES: We studied the amino acid-like compounds present in ES of L. sericata larvae in order to determine the compounds present and their potential role in the wound healing process. METHODS: These included thin-layer chromatography/mass spectrometric analysis of ES to identify amino acid-like components, a turbidometric assay to investigate their potential antibacterial activity and cell proliferation studies to investigate their potential mitogenic ability. RESULTS: Three prominent compounds were detected and identified as histidine, valinol and 3-guanidinopropionic acid. While these amino acids were not shown to exhibit antibacterial activity, a proliferative effect on the growth of human endothelial cells, but not fibroblasts, was noted. CONCLUSIONS: The demonstrated proliferative effect, selectively on endothelial cells, suggests that the amino acid-like compounds present in maggot ES may have a role in wound healing, by stimulating angiogenesis.
Authors:
A Bexfield; A E Bond; C Morgan; J Wagstaff; R P Newton; N A Ratcliffe; E Dudley; Y Nigam
Related Documents :
6735593 - Analogs of viroidin. synthesis of four virotoxin-like f-actin binding heptapeptides wit...
10794683 - Potent derivatives of glucagon-like peptide-1 with pharmacokinetic properties suitable ...
10190193 - Additional information to the in vitro antioxidant activity of ginkgo biloba l.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-03
Journal Detail:
Title:  The British journal of dermatology     Volume:  162     ISSN:  1365-2133     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-08     Completed Date:  2010-09-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  554-62     Citation Subset:  IM    
Affiliation:
School of the Environment and Society, Swansea University, Swansea SA2 8PP, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Animals
Anti-Bacterial Agents / metabolism*
Bodily Secretions / metabolism
Chromatography, Thin Layer
Diptera / metabolism*
Humans
Larva / metabolism*
Mass Spectrometry
Wound Healing / physiology*
Wound Infection / therapy*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Anti-Bacterial Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Oestradiol Protects Against the Harmful Effects of Fluoride More by Increasing Thiol Group Levels th...
Next Document:  Trends of skin diseases in organ-transplant recipients transplanted between 1966 and 2006: a cohort ...