Document Detail


Amino acid content of L5178Y and L5178Y/L-ASE cells after L-asparaginase treatment.
MedLine Citation:
PMID:  4084239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The amino acid contents of tumor cells that are either sensitive or resistant to treatment with L-asparaginase were measured. These amino acid concentrations were measured as a function of incubation time with L-asparaginase or as a function of the L-asparaginase dose. The cell types compared were the mouse leukemia lines L5178Y (sensitive to L-asparaginase treatment) and L5178Y/L-ASE (resistant to L-asparaginase treatment). Upon L-asparaginase treatment both cell lines lost most of their cellular asparagine but, whereas the resistant cells exhibited the ability to rebound to about 50% of initial values, the sensitive cells did not. While previous work had suggested that asparagine-dependent glycine synthesis was essential for sensitive cells (but not in resistant cells), we found no difference in the glycine content of either of the two cell lines as a function of either time or dose that would support this hypothesis. Major differences between the two cell lines were seen in the content of the essential amino acids before treatment with L-asparaginase. After incubation without L-asparaginase the contents of the two cell lines became similar. These results are discussed in terms of possible mechanisms of L-asparaginase sensitivity and resistance.
Authors:
J F Keefer; D A Moraga; S M Schuster
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical medicine     Volume:  34     ISSN:  0006-2944     ISO Abbreviation:  Biochem Med     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1986-02-18     Completed Date:  1986-02-18     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0151424     Medline TA:  Biochem Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  135-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Animals
Asparaginase / pharmacology*
Chromatography, High Pressure Liquid
Drug Resistance
Indicators and Reagents
Leukemia L5178 / metabolism*
Leukemia, Experimental / metabolism*
Male
Mice
Mice, Inbred DBA
Solvents
Grant Support
ID/Acronym/Agency:
CA 00628/CA/NCI NIH HHS; CA 28725/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Indicators and Reagents; 0/Solvents; EC 3.5.1.1/Asparaginase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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