Document Detail


Amino acid composition, including key derivatives of eccrine sweat: potential biomarkers of certain atopic skin conditions.
MedLine Citation:
PMID:  23075272     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The free amino acid (AA) composition of eccrine sweat is different from other biological fluids, for reasons which are not properly understood. We undertook the detailed analysis of the AA composition of freshly isolated pure human eccrine sweat, including some of the key derivatives of AA metabolism, to better understand the key biological mechanisms governing its composition. Eccrine sweat was collected from the axillae of 12 healthy subjects immediately upon formation. Free AA analysis was performed using an automatic AA analyser after ninhydrin derivatization. Pyrrolidine-5-carboxylic acid (PCA) and urocanic acid (UCA) levels were determined using GC/MS. The free AA composition of sweat was dominated by the presence of serine accounting for just over one-fifth of the total free AA composition. Glycine was the next most abundant followed by PCA, alanine, citrulline and threonine, respectively. The data obtained indicate that the AA content of sweat bears a remarkable similarity to the AA composition of the epidermal protein profilaggrin. This protein is the key source of free AAs and their derivatives that form a major part of the natural moisturizing factor (NMF) within the stratum corneum (SC) and plays a major role in maintaining the barrier integrity of human skin. As perturbations in the production of NMF can lead to abnormal barrier function and can arise as a consequence of filaggrin genotype, we propose the quantification of AAs in sweat may serve as a non-invasive diagnostic biomarker for certain atopic skin conditions, that is, atopic dermatitis (AD).
Authors:
Harker Mark; Clive R Harding
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-20
Journal Detail:
Title:  International journal of cosmetic science     Volume:  35     ISSN:  1468-2494     ISO Abbreviation:  Int J Cosmet Sci     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-11     Completed Date:  2013-09-10     Revised Date:  2013-12-10    
Medline Journal Info:
Nlm Unique ID:  8007161     Medline TA:  Int J Cosmet Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  163-8     Citation Subset:  IM    
Copyright Information:
© 2012 Society of Cosmetic Scientists and the Société Française de Cosmétologie.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Biological Markers / chemistry*
Eccrine Glands / secretion*
Humans
Skin Diseases / diagnosis*
Sweat / chemistry*,  secretion
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Biological Markers
Comments/Corrections
Erratum In:
Int J Cosmet Sci. 2013 Jun;35(3):319

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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