Document Detail

Amino acid composition of human fibrinogen and anticoagulant derivatives.
MedLine Citation:
PMID:  6056635     Owner:  NLM     Status:  MEDLINE    
1. The amino acid compositions of human fibrinogen and three intermediate anticoagulant derivatives were determined by column chromatography. The derivatives were isolated by ammonium sulphate fractionation and column electrophoresis from solutions of fibrinogen undergoing spontaneous breakdown. One derivative, isolated as the large electrophoretic peak at the end of the clottable period (100% CP) of the parent fibrinogen solution, was labelled LP(100) and others obtained at twice this period (200% CP) were designated as LP(200) and SP(200) (LP, large peak; SP, small peak). 2. Maximal ;molecular' weights of approx. 294000 for LP(100), 137000 for LP(200) and 37000 for SP(200) were calculated for the protein moieties. At least 265 amino acid residues must have been lost from each fibrinogen molecule during the formation of LP(100), and 1362 during the formation of the other two derivatives. 3. Only one derivative (LP(200)) had a partial specific volume ([unk] 0.725ml./g.) different from that of fibrinogen ([unk] 0.721ml./g.). 4. No significant differences in refractive index at 589mmu were detected. 5. Calculation of the total number of ionizable groups/10(5)g. of each protein moiety showed a preponderance of the following numbers of negative charges: 22 in fibrinogen; 24 in LP(100); 26 in LP(200); 49 in SP(200). The isoionic points were estimated to be approx.+0.03pH unit (for fibrinogen), -0.06pH unit for (LP(100)) and +0.28pH unit (for LP(200)) from the pK of imidazole, and 0.78pH unit above the average pK of aspartyl and glutamyl ions (for SP(200)). These figures agree closely with experimentally determined values of the isoelectric point of fibrinogen and its derivatives.
E Triantaphyllopoulos; D C Triantaphyllopoulos
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  105     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1967 Oct 
Date Detail:
Created Date:  1968-01-06     Completed Date:  1968-01-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  393-400     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis
Anticoagulants / analysis*
Chemistry, Physical
Fibrinogen / analysis*
Physicochemical Phenomena
Trypsin Inhibitors
Reg. No./Substance:
0/Amino Acids; 0/Anticoagulants; 0/Trypsin Inhibitors; 9001-32-5/Fibrinogen

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