Document Detail

Amino acid composition and antioxidant activities of hydrolysates and peptide fractions from porcine collagen.
MedLine Citation:
PMID:  23064526     Owner:  NLM     Status:  Publisher    
The amino acid composition and antioxidant activities of different hydrolysates from porcine collagen were analyzed. The gelatin was hydrolyzed for antioxidative peptides with various proteases, namely papain, protease from bovine pancreas, protease from Streptomyces, and cocktail mixture of protease from bovine pancreas and protease from Streptomyces. The hydrolysates were assessed using methods of DPPH radical-scavenging ability, metal-chelating ability and lipid peroxidation inhibition activity. It was found that the collagen hydrolysates by different protease treatments had different amino acid compositions and antioxidant properties. However, the contents of Hyp and Pro were improved and the content of Gly was decreased in each collagen hydrolysate compared with collagen. The hydrolysate prepared with the cocktail mixture of proteases, which exhibited the highest antioxidant activity, was separated into 6 fractions by gel filtration chromatography. Fraction 2 was further separated by ion exchange chromatography. Fraction 2b with abundant basic amino acids and Fraction 2d which was slightly acidic fractions had higher radical-scavenging and metal-chelating activities, and both Fraction 2b and 2d contained more hydrophobic amino acids. The results confirmed that the antioxidative peptides were rich in Hyp, Pro and Gly, which accounted for half of amino acid composition. This article added further support to the preparation of natural antioxidative peptides from porcine skin collagen.
Jing Ao; Bo Li
Related Documents :
24486936 - Fresh water green microalga scenedesmus abundans: a potential feedstock for high qualit...
18478426 - Studying pellet formation of a filamentous fungus rhizopus oryzae to enhance organic ac...
23435976 - Genetic examination of initial amino acid oxidation and glutamate catabolism in the hyp...
24287986 - Three new ursane-type triterpenoids from the stems of saprosma merrillii.
11223866 - Macrophages transfer [14c]-labelled fatty acids to pancreatic islets in culture.
9676266 - Distribution of free d-amino acids in tissues and body fluids of vertebrates.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-11
Journal Detail:
Title:  Food science and technology international = Ciencia y tecnologia de los alimentos internacional     Volume:  -     ISSN:  1082-0132     ISO Abbreviation:  Food Sci Technol Int     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889534     Medline TA:  Food Sci Technol Int     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
College of Food Science and Nutritional Engineering, China Agricultural University.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Optimization of process for reduction of antinutritional factors in edible cereal brans.
Next Document:  The quantum free energy barrier for hydrogen vacancy diffusion in Na(3)AlH(6).