Document Detail


Amino acid composition of Clostridium botulinum type B neurotoxin.
MedLine Citation:
PMID:  6729847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To characterize type B botulinum neurotoxin based on reliable data on the amino acid composition, three batches of the neurotoxin were analyzed. Each batch was isolated from a separate neurotoxin producing bacterial culture (strain Okra). Two batches were purified by the same method and one was purified by a different method. The toxin preparations were comparable in purity (judged by polyacrylamide gel--sodium dodecyl sulfate electrophoresis) and similar in amino acid composition. The best estimate of the number of amino acid residues per toxin molecule (mol. wt 152,000) was: Asp212,Thr54,Ser83,Glu130,Pro46,Gly61++ +,Ala44,Val54,CyS11,Met23,Ile144,Leu107 , Tyr81,Phe77,Lys118,His7,Arg39,Trp18.
Authors:
B R DasGupta; M A Woody
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  22     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1984  
Date Detail:
Created Date:  1984-07-02     Completed Date:  1984-07-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  312-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Botulinum Toxins*
Chemical Phenomena
Chemistry
Hydrolysis
Neurotoxins*
Grant Support
ID/Acronym/Agency:
NS17742/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Botulinum Toxins; 0/Neurotoxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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