Document Detail

Amino acid composition of Clostridium botulinum type B neurotoxin.
MedLine Citation:
PMID:  6729847     Owner:  NLM     Status:  MEDLINE    
To characterize type B botulinum neurotoxin based on reliable data on the amino acid composition, three batches of the neurotoxin were analyzed. Each batch was isolated from a separate neurotoxin producing bacterial culture (strain Okra). Two batches were purified by the same method and one was purified by a different method. The toxin preparations were comparable in purity (judged by polyacrylamide gel--sodium dodecyl sulfate electrophoresis) and similar in amino acid composition. The best estimate of the number of amino acid residues per toxin molecule (mol. wt 152,000) was: Asp212,Thr54,Ser83,Glu130,Pro46,Gly61++ +,Ala44,Val54,CyS11,Met23,Ile144,Leu107 , Tyr81,Phe77,Lys118,His7,Arg39,Trp18.
B R DasGupta; M A Woody
Related Documents :
6214397 - Mechanism of tumor and liver concentration of 111in and 169yb: 111in and 169yb binding ...
16403427 - Detection of dimethylarginines in protein hydrolysates by matrix-assisted laser desorpt...
3731487 - Separation of apolipoprotein b subfractions by high performance gel permeation chromato...
465537 - Isolation and partial characterization of the major glycoprotein from the plasma membra...
22163027 - Dietary essential amino acids affect the reproduction of the keystone herbivore daphnia...
1753217 - Site-specific methionine sulfoxide formation is the structural basis of chromatographic...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  22     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1984  
Date Detail:
Created Date:  1984-07-02     Completed Date:  1984-07-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  312-5     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / analysis*
Botulinum Toxins*
Chemical Phenomena
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Botulinum Toxins; 0/Neurotoxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Ultrastructural study of the local changes induced in the white rat by the venom of Bothrops picadoi...
Next Document:  Radiation exposure in high resolution computed tomography of the spine.