| Amino acid composition of Clostridium botulinum type B neurotoxin. | |
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MedLine Citation:
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PMID: 6729847 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To characterize type B botulinum neurotoxin based on reliable data on the amino acid composition, three batches of the neurotoxin were analyzed. Each batch was isolated from a separate neurotoxin producing bacterial culture (strain Okra). Two batches were purified by the same method and one was purified by a different method. The toxin preparations were comparable in purity (judged by polyacrylamide gel--sodium dodecyl sulfate electrophoresis) and similar in amino acid composition. The best estimate of the number of amino acid residues per toxin molecule (mol. wt 152,000) was: Asp212,Thr54,Ser83,Glu130,Pro46,Gly61++ +,Ala44,Val54,CyS11,Met23,Ile144,Leu107 , Tyr81,Phe77,Lys118,His7,Arg39,Trp18. |
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Authors:
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B R DasGupta; M A Woody |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: 22 ISSN: 0041-0101 ISO Abbreviation: Toxicon Publication Date: 1984 |
Date Detail:
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Created Date: 1984-07-02 Completed Date: 1984-07-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 312-5 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Botulinum Toxins* Chemical Phenomena Chemistry Hydrolysis Neurotoxins* |
| Grant Support | |
ID/Acronym/Agency:
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NS17742/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Botulinum Toxins; 0/Neurotoxins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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