Document Detail


Amino acid change 335 E to K affects the sialic-acid-binding and neuraminidase activities of Urabe AM9 mumps virus hemagglutinin-neuraminidase glycoprotein.
MedLine Citation:
PMID:  17223599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A mutation coding for the amino acid change E335 to K is frequently found in the hemagglutinin-neuraminidase (HN) gene of Urabe AM9 mumps viruses isolated during post-vaccination meningitis cases. To identify if this mutation modifies the biological activities of the HN glycoprotein, two variants of Urabe AM9 vaccine differing at amino acid 335 (HN-E335 and HN-K335) were isolated and their receptor-binding specificity was determined by means of competence assays. Pre-incubation of the viruses with sialic acids inhibited both syncytia formation in Vero cells and replication in SH-SY5Y cells. Thus, HN-K335 showed higher affinity towards sialylalpha2,6lactose, whereas HN-G335 preferred sialylalpha2,3lactose. These results are relevant because a high expression of sialylalpha2,6lactose in nerve cells was confirmed by means of Sambucus nigra lectin-cytochemistry. In addition, kinetics assays showed that HN-K335 and HN-E335 also differ in their hydrolysis rate (Vmax values of 37.5 vs. 3.5 nmol min-1mg-1, respectively). Therefore, HN-K335 variant presented a neuraminidase activity level 11-fold higher than that of HN-E335 variant. In conclusion, the mutation affects the receptor-binding and neuraminidase activities of Urabe AM9 mumps virus variants.
Authors:
Julio Reyes-Leyva; Rocío Baños; María Borraz-Argüello; Gerardo Santos-López; Nora Rosas; Gabriela Alvarado; Irma Herrera; Verónica Vallejo; José Tapia-Ramírez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-18
Journal Detail:
Title:  Microbes and infection / Institut Pasteur     Volume:  9     ISSN:  1286-4579     ISO Abbreviation:  Microbes Infect.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-16     Completed Date:  2007-08-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883508     Medline TA:  Microbes Infect     Country:  France    
Other Details:
Languages:  eng     Pagination:  234-40     Citation Subset:  IM    
Affiliation:
Laboratorio de Virología y Biología Molecular, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Mexico. jreyesleyva@correo.unam.mx
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Substitution*
Animals
Cell Line
Cercopithecus aethiops
HN Protein / chemistry,  genetics*,  metabolism*
Humans
Mumps virus / genetics,  physiology*
Mutation, Missense
N-Acetylneuraminic Acid / metabolism*
Receptors, Virus / metabolism*
Virus Attachment*
Chemical
Reg. No./Substance:
0/HN Protein; 0/Receptors, Virus; 131-48-6/N-Acetylneuraminic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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