Document Detail

Amino-acid-dependent shift in tRNA synthetase editing mechanisms.
MedLine Citation:
PMID:  22017352     Owner:  NLM     Status:  MEDLINE    
Many aminoacyl-tRNA synthetases prevent mistranslation by relying upon proofreading activities at multiple stages of the aminoacylation reaction. In leucyl-tRNA synthetase (LeuRS), editing activities that precede or are subsequent to tRNA charging have been identified. Although both are operational, either the pre- or post-transfer editing activity can predominate. Yeast cytoplasmic LeuRS (ycLeuRS) misactivates structurally similar noncognate amino acids including isoleucine and methionine. We show that ycLeuRS has a robust post-transfer editing activity that efficiently clears tRNA(Leu) mischarged with isoleucine. In comparison, the enzyme's post-transfer hydrolytic activity against tRNA(Leu) mischarged with methionine is weak. Rather, methionyl-adenylate is cleared robustly via an enzyme-mediated pre-transfer editing activity. We hypothesize that, similar to E. coli LeuRS, ycLeuRS has coexisting functional pre- and post-transfer editing activities. In the case of ycLeuRS, a shift between the two editing pathways is triggered by the identity of the noncognate amino acid.
Jaya Sarkar; Susan A Martinis
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-10-31
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  133     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-17     Completed Date:  2012-03-12     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18510-3     Citation Subset:  IM    
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MeSH Terms
Amino Acids* / genetics,  metabolism
Amino Acyl-tRNA Synthetases / chemistry,  genetics,  metabolism*
Escherichia coli / enzymology
RNA, Transfer, Leu*
Yeasts / enzymology
Grant Support
GM063789/GM/NIGMS NIH HHS; R01 GM063789/GM/NIGMS NIH HHS; R01 GM063789-07S1/GM/NIGMS NIH HHS; R01 GM063789-08/GM/NIGMS NIH HHS; R01 GM063789-09/GM/NIGMS NIH HHS; R01 GM063789-10/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Amino Acids; 0/RNA, Transfer, Leu; EC 6.1.1.-/Amino Acyl-tRNA Synthetases

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