| Amino Acid analysis by hydrophilic interaction chromatography coupled with isotope dilution mass spectrometry. | |
| | |
MedLine Citation:
|
PMID: 22125136 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Here, we describe an amino acid analysis that is based on the use of hydrophilic interaction liquid chromatography coupled with isotope dilution mass spectrometry for the accurate quantification of underivatized amino acids from hydrolyzed peptide/protein. Twelve underivatized amino acids were separated and detected during an 88-min runtime. The absolute limits of detection and limits of quantification (on column) of the four amino acids (isoleucine, phenylalanine, proline, and valine) were in the range of 6-80 and 20-200 fmol, respectively. As little as 25 pmol of peptide or protein hydrolysates is sufficient for determining absolute content. |
| | |
Authors:
|
Megumi Kato; Akiko Takatsu |
Related Documents
:
|
19938836 - Proline-beta(3)-amino-ester dipeptides as efficient catalysts for enantioselective dire... 21688306 - Dissipation of six acid herbicides in water and sediment of two canadian prairie wetlands. 20560576 - A lewis acid promoted oxidative cyclization. 21983406 - Synthesis of fatty acid methyl ester from palm oil (elaeis guineensis) with k(y)(mgca)(... 3488466 - Chloride transport in bullfrog gastric mucosa. 15122306 - Asbestos-derived reactive oxygen species activate tgf-beta1. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 828 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
|
Created Date: 2011-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 55-62 Citation Subset: IM |
Affiliation:
|
Bio-Medical Standard Section, National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan, katou-megu@aist.go.jp. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Amino Acid Analysis via LC-MS Method After Derivatization with Quaternary Phosphonium.
Next Document: A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids.