Document Detail


An Amino Acid Mixture Mitigates Radiation-induced Gastrointestinal Toxicity.
MedLine Citation:
PMID:  24776907     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Electrolyte and nutrient absorption occur in villous epithelial cells. Radiation often results in reduced electrolyte and nutrient absorption, which leads to gastrointestinal toxicity. Therefore, the authors studied: (1) radiation-induced changes in glucose and amino acid absorption across ileal tissues and (2) the effect of amino acid mixtures on absorptive capacity. NIH Swiss mice were irradiated (0, 1, 3, 5, or 7 Gy) using a Cs source at 0.9 Gy min. Transepithelial short circuit current (Isc), dilution potential, and isotope flux determinations were made in Ussing chamber studies and correlated to plasma endotoxin and IL-1β levels. Amino acids that increased electrolyte absorption and improved mucosal barrier functions were used to create a mitigating amino acid mixture (MAAM). The MAAM was given to mice via gastric gavage; thereafter, body weight and survival were recorded. A significant decrease in basal and glucose-stimulated sodium absorption occurred after 0, 1, 3, 5, and 7 Gy irradiation. Ussing chamber studies showed that paracellular permeability increased following irradiation and that the addition of glucose resulted in a further increase in permeability. Following irradiation, certain amino acids manifested decreased absorption, whereas others were associated with increased absorption. Lysine, aspartic acid, glycine, isoleucine, threonine, tyrosine, valine, tryptophan, and serine decreased plasma endotoxins were selected for the MAAM. Mice treated with the MAAM showed increased electrolyte absorption and decreased paracellular permeability, IL-1β levels, and plasma endotoxin levels. Mice treated with MAAM also had increased weight gain and better survival following irradiation. The MAAM has immediate potential for use in mitigating radiation-induced acute gastrointestinal syndrome.
Authors:
Liangjie Yin; Pooja Vijaygopal; Rejeesh Menon; Lauren A Vaught; Mei Zhang; Lurong Zhang; Paul Okunieff; Sadasivan Vidyasagar
Related Documents :
14633047 - Modulation of na+ transport across isolated rumen epithelium by short-chain fatty acids...
22860007 - Rapid one-step selection method for generating nucleic acid aptamers: development of a ...
22919677 - Lactic acid bacteria contribution to gut microbiota complexity: lights and shadows.
15377617 - The passage of lactic acid bacteria from silage into rumen fluid, in vitro studies.
10575097 - Melittin enhances amino acid and free fatty acid release from the in vivo cerebral cortex.
6337177 - Effect of in vitro action of serum proteases or exposure to acid on measurable immunore...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Health physics     Volume:  106     ISSN:  1538-5159     ISO Abbreviation:  Health Phys     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-04-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985093R     Medline TA:  Health Phys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  734-44     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Significance of bioindicators to predict survival in irradiated minipigs.
Next Document:  Cytokinesis-block Micronucleus Assay by Manual and Automated Scoring: Calibration Curves and Dose Pr...