| Amino Acid Analysis via LC-MS Method After Derivatization with Quaternary Phosphonium. | |
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MedLine Citation:
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PMID: 22125135 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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(5-N-Succinimidoxy-5-oxopentyl)triphenylphosphonium bromide (SPTPP) is a highly sensitive and positively charged precolumn derivatization reagent for the analysis of amino acids in liquid chromatography-electrospray ionization-tandem mass spectrometry. The synthesis of this reagent and handling of the derivatization reaction are quite simple. It reacts with amino acids rapidly and with high efficiency. MS/MS analysis revealed that the SPTPP-derivatized amino acids formed strong product ions; thus, highly sensitive and selective detection is possible in the selected reaction monitoring mode. The limits of detection for the SPTPP-derivatized amino acids are in the sub-fmol range. The sensitivities of the derivatized amino acids increased about 500-fold, as compared to those of underivatized amino acids. |
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Authors:
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Shinsuke Inagaki; Toshimasa Toyo'oka |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 828 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 47-54 Citation Subset: IM |
Affiliation:
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Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, and Global COE Program, University of Shizuoka, Shizuoka, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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