Document Detail

Amiloride in differentiation and commitment of Friend erythroleukemia cells.
MedLine Citation:
PMID:  3862669     Owner:  NLM     Status:  MEDLINE    
Dimethylsulfoxide (DMSO) converts almost all of the undifferentiated murine erythroleukemia cells (MEL or Friend cells, clone 745A) in a culture to differentiated cells that contain high levels of hemoglobin and that stop growing after a limited number of cell divisions. Contrary to other reports--that amiloride strongly inhibits DMSO-induced differentiation in MEL cells--in this laboratory, inhibition by amiloride, tested with DMSO over a range of concentrations in two kinds of media and at various cell densities, was found to be only weak or absent. Similarly, amiloride did not inhibit induction by N,N'-hexamethylene bis-acetamide (HMBA). As expected from previous findings with other cell systems, amiloride inhibited protein synthesis and cell multiplication.
M Lubin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  124     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-11-21     Completed Date:  1985-11-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  539-44     Citation Subset:  IM    
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MeSH Terms
Acetamides / pharmacology
Amiloride / pharmacology*
Benzidines / analysis
Cell Differentiation / drug effects
Cell Division / drug effects
Cell Line
Dimethyl Sulfoxide / pharmacology
Friend murine leukemia virus
Hemoglobins / analysis
Leukemia, Erythroblastic, Acute / ultrastructure*
Leukemia, Experimental / pathology*
Protein Biosynthesis
Reg. No./Substance:
0/Acetamides; 0/Benzidines; 0/Hemoglobins; 2609-46-3/Amiloride; 3073-59-4/hexamethylene bisacetamide; 67-68-5/Dimethyl Sulfoxide; 92-87-5/benzidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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