Document Detail

Amelogenin Enhances the Proliferation of Cementoblast Lineage Cells.
MedLine Citation:
PMID:  21486179     Owner:  NLM     Status:  Publisher    
Background: It is well known that enamel matrix proteins play a crucial role in tooth root formation as well as amelogenesis. Since amelogenin is a major enamel matrix protein, it is assumed that amelogenin also affects the metabolism of cementum. However, the biological functions of amelogenin in cementoblasts remain unclear. The purpose of this study was to examine the effect of recombinant human full-length amelogenin (rh174) on the proliferation of cultured human cementoblast-like (HCEM) and periodontal ligament (HPDL) cells. Methods: HCEM and HPDL cells were cultured and treated with 100 ng/ml rh174 in the presence or absence of an anti-cluster of differentiation (CD) 63 blocking antibody. Cell proliferation was evaluated using a cell proliferation ELISA BrdU kit and quantification of the cell number by 3-(4, 5-dimethylthiazol-2-yl)-5-(3-car-boxymethoxyphenyl)-2-(4-sulfophenyl)-2 H-tetrazolium-inner salt (MTS) assay. The phosphorylation of extracellular signal regulated kinases (ERK) 1/2 was measured by ELISA and Western blot analysis. Results: The proliferation of HCEM and HPDL cells was enhanced significantly (p<0.05) by treatment with rh174, and inhibited significantly (p<0.05) by the addition of anti-CD63 blocking antibody. In addition, the ratio of phosphorylated ERK1/2 to total ERK1/2 became significantly larger (p<0.05) by treatment with rh174, and was reduced significantly by the addition of anti-CD63 blocking antibody in both HCEM and HPDL cells. Conclusion: These results showed that rh174 interacts with CD63, and rh174/CD63 interaction activates the ERK1/2 signaling pathway, enhancing the proliferation activities of HCEM and HPDL cells.
Ryo Kunimatsu; Kotaro Tanimoto; Yuki Tanne; Takashi Kamiya; Satoru Ohkuma; Yu-Ching Huang; Yuki Yoshimi; Mutsumi Miyauchi; Takashi Takata; Kazuo Tanne
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-12
Journal Detail:
Title:  Journal of periodontology     Volume:  -     ISSN:  1943-3670     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000345     Medline TA:  J Periodontol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences.
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