Document Detail

Amelioration of altered antioxidant status and membrane linked functions by vanadium and Trigonella in alloxan diabetic rat brains.
MedLine Citation:
PMID:  16184010     Owner:  NLM     Status:  MEDLINE    
Trigonella foenum graecum seed powder (TSP) and sodium orthovanadate (SOV) have been reported to have antidiabetic effects. However, SOV exerts hypoglycemic effects at relatively high doses with several toxic effects. We used low doses of vanadate in combination with TSP and evaluated their antidiabetic effects on anti-oxidant enzymes and membrane-linked functions in diabetic rat brains. In rats, diabetes was induced by alloxan monohydrate (15 mg/100 g body wt.) and they were treated with 2 IU insulin, 0.6 mg/ml SOV, 5% TSP and a combination of 0.2 mg/ml SOV with 5% TSP for 21 days. Blood glucose levels, activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), Na+/K+ ATPase, membrane lipid peroxidation and fluidity were determined in different fractions of whole brain after 21 days of treatment. Diabetic rats showed high blood glucose (P less than 0.001), decreased activities of SOD, catalase and Na+/K+ ATPase (P less than 0.01, P less than 0.001 and P less than 0.01), increased levels of GPx and MDA (P less than 0.01 and P less than 0.001) and decreased membrane fluidity (P less than 0.01). Treatment with different antidiabetic compounds restored the above-altered parameters. Combined dose of Trigonella and vanadate was found to be the most effective treatment in normalizing these alterations. Lower doses of vanadate could be used in combination with TSP to effectively counter diabetic alterations without any toxic effects.
Mohammad Rizwan Siddiqui; Asia Taha; K Moorthy; Mohd Ejaz Hussain; S F Basir; Najma Zaheer Baquer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biosciences     Volume:  30     ISSN:  0250-5991     ISO Abbreviation:  J. Biosci.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-26     Completed Date:  2008-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100809     Medline TA:  J Biosci     Country:  India    
Other Details:
Languages:  eng     Pagination:  483-90     Citation Subset:  IM    
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
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MeSH Terms
Brain / drug effects,  metabolism*
Cell Membrane / drug effects*
Diabetes Mellitus, Experimental / drug therapy,  metabolism
Lipid Peroxidation / drug effects
Oxidative Stress / drug effects*
Rats, Wistar
Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors,  metabolism
Trace Elements / pharmacology*
Trigonella / metabolism*
Vanadates / pharmacology
Vanadium / pharmacology*
Reg. No./Substance:
0/Trace Elements; 0/Vanadates; 7440-62-2/Vanadium; EC ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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