Document Detail


Ambient particulate matter causes activation of the c-jun kinase/stress-activated protein kinase cascade and DNA synthesis in lung epithelial cells.
MedLine Citation:
PMID:  9788597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Numerous epidemiological studies have demonstrated a positive association between ambient air pollution and adverse health effects including respiratory morbidity, asthma, and lung cancer. It has been suggested in some experimental studies that airborne particulate matter (PM) can produce inflammatory effects, but nothing is known about the possible proliferative and carcinogenic effects of these particles on cells of the lung. We show here that exposure of pulmonary epithelial cells, a cell type affected in acute lung injury, asthma, and lung carcinomas, to nontoxic concentrations of PM in vitro results in increases in c-jun kinase activity, levels of phosphorylated cJun immunoreactive protein, and transcriptional activation of activator protein-1-dependent gene expression. These changes are accompanied by elevations in numbers of cells incorporating 5'-bromodeoxyuridine, a marker of unscheduled DNA synthesis and/or cell proliferation. Data here are the first to demonstrate that interaction of ambient PM with target cells of the lung initiates a cell signaling cascade related causally to aberrant cell proliferation and carcinogenesis.
Authors:
C Timblin; K BeruBe; A Churg; K Driscoll; T Gordon; D Hemenway; E Walsh; A B Cummins; P Vacek; B Mossman
Related Documents :
21706477 - Ribosomal protein rpl41 induces rapid degradation of atf4, a transcription factor criti...
22249267 - Diversity within the prb pathway: is there a code of conduct?
6471147 - Ultrastructure of bronchopulmonary lavage cells from bovines administered 3-methylindol...
3322867 - The origins of secretions in the lower respiratory tract.
12025407 - Disruption of mitochondria is an early event during dolichyl monophosphate-induced apop...
22863707 - Alkylphenols from the roots of ardisia brevicaulis induce g1 arrest and apoptosis throu...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  58     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-11-06     Completed Date:  1998-11-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4543-7     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Vermont, Burlington 05405, USA. ctimblin@zoo.uvm.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Air Pollution / adverse effects*
Animals
Bromodeoxyuridine / metabolism
Calcium-Calmodulin-Dependent Protein Kinases / physiology*
Cell Division
DNA / biosynthesis*
Enzyme Activation
Epithelial Cells / metabolism
JNK Mitogen-Activated Protein Kinases
Lung / metabolism*
Mitogen-Activated Protein Kinases*
Phosphorylation
Rats
Transcription Factor AP-1 / physiology
Grant Support
ID/Acronym/Agency:
ES0649/ES/NIEHS NIH HHS; ES09213/ES/NIEHS NIH HHS; HL-39469/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Transcription Factor AP-1; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Human exonuclease I interacts with the mismatch repair protein hMSH2.
Next Document:  Inhibition by phenyl N-tert-butyl nitrone of early phase carcinogenesis in the livers of rats fed a ...