| Alzheimer paired helical filaments (PHFs) studied by high-resolution TEM: what can vertical Pt-C replication tell us about the organization of the pronase-digested PHF core? | |
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MedLine Citation:
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PMID: 16103996 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Untreated paired helical filaments (PHFs) and pronase-digested PHF-core filaments were stereoscopically imaged with a freeze-drying vertical platinum-carbon replication preparation method for TEM. The untreated PHF have an average wide region (W) = 22.8 +/- 2.4 nm, a narrow region (T) = 10.6 +/- 1.7 nm, and a helical turn period (L) = 78.6 +/- 13.4. The surfaces of the untreated PHF's fuzzy coat appears disorganized. The widths of the pronase-treated PHF-core filaments were significantly reduced (W(d) = 14.8 +/- 1.2 nm, T(d) = 5.7 +/- 1.0 nm, and L(d) = 75.4 +/- 17 nm). The surfaces of the untreated PHF contained approximately 1.1 nm strands, the same size as tau monomer ( approximately 1.0 nm). The pronase-digested PHF cores mostly contained approximately 1.6 +/- 0.3 nm strands although strand diameters ranged from 0.6-2.5 nm. The strands sometimes appear to be wrapped around the filament axis; less often, they appear to be roughly parallel to the PHF axis, and otherwise appear to be randomly oriented. Images of pronase-digested PHF core images are discussed in relation to the core's biochemical composition, its proposed beta structure, and structural subunit models. Images of the untreated and the pronase-digested PHF support a helical ribbon morphology. |
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Authors:
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George C Ruben; Michal Novak; Patricia C Edwards; Khalid Iqbal |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Microscopy research and technique Volume: 67 ISSN: 1059-910X ISO Abbreviation: Microsc. Res. Tech. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-08-22 Completed Date: 2006-01-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9203012 Medline TA: Microsc Res Tech Country: United States |
Other Details:
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Languages: eng Pagination: 196-209 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA. George.C.Ruben@Dartmouth.Edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alzheimer Disease
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pathology* Freeze Drying Humans Male Microscopy, Electron, Transmission Middle Aged Neurofibrillary Tangles / chemistry, ultrastructure* Pronase / chemistry Replica Techniques |
| Grant Support | |
ID/Acronym/Agency:
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AG05892/AG/NIA NIH HHS; AG11054/AG/NIA NIH HHS; NS18105/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.4.24.-/Pronase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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