| Alveolar hydatid cyst (AHC): inflammation-induced reactive gastrointestinal (GL) amyloidosis in AHC-infected mice and chemical characterization of the GL amyloid. | |
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MedLine Citation:
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PMID: 8654537 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A high incidence of GI amyloidosis has been described in patients with various forms of systemic amyloidosis but its evolution and progression in different subregions of the GI tract are not well documented. These aspects including the chemical nature of GI amyloid were examined in the AHC mouse model of inflammation-associated reactive amyloidosis. C57BL/6 mice were infected intraperitoneally with 250 AHC. Paraffin sections from the stomach and the small and large intestines of AHC mice were stained at different time intervals with Congo red or immunocytochemically with monospecific RAA. The submucosal blood vessels at 1 week postinfection were found to be the first target of amyloid deposition. With time the amyloid deposits extended to the mucosa and the Peyer's patches and immunoreacted with RAA; ileum was the most severely affected region. Amyloid was extracted from the GI tract and purified by size exclusion chromatography using 5 M guanidine-formic acid, pH 3. The purified amyloid was identified by Western blotting using RAA and by partial N-terminal microsequencing up to 10 cycles. The GI amyloid showed homology with murine SAA2, although SAA2 mRNA is not expressed in murine GI tract. These results shows that (a) the GI amyloid is derived, similar to that of splenic/hepatic amyloid, from circulating SAA2 and (b) the GI tract submucosal blood vessels are the first target of AA deposition. The data also suggest that AA-mediated damage to the submucosal blood capillaries may lead to SAA leakage followed by cascading of AA deposition in other layers of the GI tract. |
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Authors:
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W Li; S L Chan; S Chronopoulos; A Bell; Z Ali-Khan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental parasitology Volume: 83 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-08-01 Completed Date: 1996-08-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1-10 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amyloidosis / etiology*, metabolism Animals Blotting, Western Disease Models, Animal Echinococcosis / complications* Electrophoresis, Polyacrylamide Gel Gastrointestinal Diseases / etiology*, metabolism Immunohistochemistry Intestine, Large / blood supply, chemistry, pathology Intestine, Small / blood supply, chemistry, pathology Male Mice Mice, Inbred C57BL Molecular Sequence Data Peyer's Patches / chemistry Sequence Homology, Amino Acid Serum Amyloid A Protein / analysis*, chemistry, isolation & purification Stomach / blood supply, chemistry |
| Chemical | |
Reg. No./Substance:
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0/Serum Amyloid A Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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