| Alveolar epithelial injury and pleural empyema in acute P. aeruginosa pneumonia in anesthetized rabbits. | |
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MedLine Citation:
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PMID: 8282618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We developed an experimental model of acute Pseudomonas aeruginosa pneumonia in anesthetized ventilated rabbits to determine whether bacterial-induced injury to the alveolar epithelium would occur and the effect of the injury on the pleural space. Dose-response studies established that 10(9) colony-forming units of P. aeruginosa (wild-type strain, PAO-1) were required to injure the epithelial barrier and to cause pleural empyema with exudative pleural effusions that contained both the instilled alveolar protein tracer and P. aeruginosa. We explored the mechanisms of P. aeruginosa-induced lung and pleural injury by using three isogenic bacterial strains to compare several extracellular virulence products. PAO-S21, which carries an insertion mutation in a regulatory gene that prevents the production of exoenzyme S, resulted in no lung or pleural injury. PAO-R1, which carries a deletion in a regulatory gene that controls the production of elastase and alkaline protease, caused the same degree of lung and pleural injury as PAO-1 did. Instillation of PLC-SRN, which has both structural genes encoding phospholipase C activity deleted, resulted in a moderate reduction in alveolar epithelial injury. Although other products may be involved, exoenzyme S and phospholipase C are important in mediating injury to the alveolar epithelial barrier in acute P. aeruginosa pneumonia in rabbits. |
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Authors:
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J P Wiener-Kronish; T Sakuma; I Kudoh; J F Pittet; D Frank; L Dobbs; M L Vasil; M A Matthay |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 75 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1994-02-14 Completed Date: 1994-02-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1661-9 Citation Subset: IM |
Affiliation:
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Department of Anesthesia, University of California at San Francisco 94143-0130. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anesthesia Animals Blood-Air Barrier / physiology Empyema, Pleural / etiology, pathology* Epithelium / pathology Extravascular Lung Water / physiology Lung / pathology Microscopy, Electron Pleura / pathology Pneumonia / complications, pathology* Pseudomonas Infections / complications, pathology* Pseudomonas aeruginosa / enzymology, genetics, metabolism Pulmonary Alveoli / pathology* Pulmonary Circulation / physiology Rabbits Respiration, Artificial Serum Albumin, Radio-Iodinated |
| Grant Support | |
ID/Acronym/Agency:
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HL-141958/HL/NHLBI NIH HHS; HL-19155/HL/NHLBI NIH HHS; HL-25816/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Serum Albumin, Radio-Iodinated |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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