Document Detail

Alveolar epithelial injury and pleural empyema in acute P. aeruginosa pneumonia in anesthetized rabbits.
MedLine Citation:
PMID:  8282618     Owner:  NLM     Status:  MEDLINE    
We developed an experimental model of acute Pseudomonas aeruginosa pneumonia in anesthetized ventilated rabbits to determine whether bacterial-induced injury to the alveolar epithelium would occur and the effect of the injury on the pleural space. Dose-response studies established that 10(9) colony-forming units of P. aeruginosa (wild-type strain, PAO-1) were required to injure the epithelial barrier and to cause pleural empyema with exudative pleural effusions that contained both the instilled alveolar protein tracer and P. aeruginosa. We explored the mechanisms of P. aeruginosa-induced lung and pleural injury by using three isogenic bacterial strains to compare several extracellular virulence products. PAO-S21, which carries an insertion mutation in a regulatory gene that prevents the production of exoenzyme S, resulted in no lung or pleural injury. PAO-R1, which carries a deletion in a regulatory gene that controls the production of elastase and alkaline protease, caused the same degree of lung and pleural injury as PAO-1 did. Instillation of PLC-SRN, which has both structural genes encoding phospholipase C activity deleted, resulted in a moderate reduction in alveolar epithelial injury. Although other products may be involved, exoenzyme S and phospholipase C are important in mediating injury to the alveolar epithelial barrier in acute P. aeruginosa pneumonia in rabbits.
J P Wiener-Kronish; T Sakuma; I Kudoh; J F Pittet; D Frank; L Dobbs; M L Vasil; M A Matthay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  75     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1994-02-14     Completed Date:  1994-02-14     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1661-9     Citation Subset:  IM    
Department of Anesthesia, University of California at San Francisco 94143-0130.
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MeSH Terms
Blood-Air Barrier / physiology
Empyema, Pleural / etiology,  pathology*
Epithelium / pathology
Extravascular Lung Water / physiology
Lung / pathology
Microscopy, Electron
Pleura / pathology
Pneumonia / complications,  pathology*
Pseudomonas Infections / complications,  pathology*
Pseudomonas aeruginosa / enzymology,  genetics,  metabolism
Pulmonary Alveoli / pathology*
Pulmonary Circulation / physiology
Respiration, Artificial
Serum Albumin, Radio-Iodinated
Grant Support
Reg. No./Substance:
0/Serum Albumin, Radio-Iodinated

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