Document Detail


Alveolar epithelial dynamics in postpneumonectomy lung growth.
MedLine Citation:
PMID:  23408540     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intimate anatomic and functional relationship between epithelial cells and endothelial cells within the alveolus suggests the likelihood of a coordinated response during postpneumonectomy lung growth. To define the population dynamics and potential contribution of alveolar epithelial cells to alveolar angiogenesis, we studied alveolar Type II and I cells during the 21 days after pneumonectomy. Alveolar Type II cells were defined and isolated by flow cytometry using a CD45(-) , MHC class II(+) , phosphine(+) phenotype. These phenotypically defined alveolar Type II cells demonstrated an increase in cell number after pneumonectomy; the increase in cell number preceded the increase in Type I (T1α(+) ) cells. Using a parabiotic wild type/GFP pneumonectomy model, <3% of the Type II cells and 1% of the Type I cells were positive for GFP-a finding consistent with the absence of a blood-borne contribution to alveolar epithelial cells. The CD45(-) , MHC class II(+) , phosphine(+) Type II cells demonstrated the active transcription of angiogenesis-related genes both before and after pneumonectomy. When the Type II cells on Day 7 after pneumonectomy were compared to nonsurgical controls, 10 genes demonstrated significantly increased expression (P<0.05). In contrast to the normal adult Type II cells, there was notable expression of inflammation-associated genes (Ccl2, Cxcl2, Ifng) as well as genes associated with epithelial growth (Ereg, Lep). Together, the data suggest an active contribution of local alveolar Type II cells to alveolar growth.
Authors:
Kenji Chamoto; Barry C Gibney; Maximilian Ackermann; Grace S Lee; Moritz A Konerding; Akira Tsuda; Steven J Mentzer
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Anatomical record (Hoboken, N.J. : 2007)     Volume:  296     ISSN:  1932-8494     ISO Abbreviation:  Anat Rec (Hoboken)     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-14     Completed Date:  2013-07-29     Revised Date:  2014-03-06    
Medline Journal Info:
Nlm Unique ID:  101292775     Medline TA:  Anat Rec (Hoboken)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  495-503     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD45 / metabolism
Biological Markers / metabolism
Cell Proliferation*
Cell Separation / methods
Flow Cytometry
Gene Expression Regulation
Green Fluorescent Proteins / biosynthesis,  genetics
Histocompatibility Antigens Class II / metabolism
Inflammation / genetics
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Electron, Transmission
Neovascularization, Physiologic / genetics
Parabiosis
Phenotype
Phosphines / metabolism
Pneumocytes* / immunology,  metabolism,  ultrastructure
Pneumonectomy*
Pulmonary Alveoli / blood supply,  growth & development*,  immunology,  metabolism,  surgery*,  ultrastructure
Regeneration* / genetics
Time Factors
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
HL007734/HL/NHLBI NIH HHS; HL75426/HL/NHLBI NIH HHS; HL94567/HL/NHLBI NIH HHS; R01 HL075426/HL/NHLBI NIH HHS; R01 HL094567/HL/NHLBI NIH HHS; T32 HL007734/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Histocompatibility Antigens Class II; 0/Phosphines; 147336-22-9/Green Fluorescent Proteins; 7803-51-2/phosphine; EC 3.1.3.48/Antigens, CD45; EC 3.1.3.48/Ptprc protein, mouse
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Penile Anatomy and Hypotheses of Erectile Function in the American Alligator (Alligator mississippie...
Next Document:  Comparative anatomy of mouse and human nail units.